Human KRT14 Recombinant Protein – Cat# MBS142903

Cytokeratin 14 (KRT14) Human Recombinant Protein

Name: KRT14 Recombinant Protein; Cytokeratin 14; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142903
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142903

Description: Cytokeratin 14 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton. The KRT14 is purified by proprietary chromatographic techniques.Introduction: Cytokeratin 14 is a member… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 14 (KRT14), Recombinant Protein

Full Product Name

Recombinant Human Cytokeratin 14

Product Gene Name

KRT14 recombinant protein

Product Synonym Names

KRT14 Human; Cytokeratin 14 Human Recombinant; Keratin; type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT14; NFJ; CK14; EBS3; EBS4

Host

E Coli

Form/Format

The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized KRT14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution KRT14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

51,561 Da

OMIM

125595

3D Structure

ModBase 3D Structure for P02533

Solubility

It is recommended to reconstitute the lyophilized CK-14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Reconstitution

Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

Product Categories/Family for KRT14 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Cytokeratin

Other Notes

Small volumes of KRT14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT14 purchase

MBS142903 is a recombinant protein targeting KRT14. Also known as Recombinant Human Cytokeratin 14. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.

NCBI GI #

15431310

NCBI GeneID

3861

NCBI Accession #

NP_000517.2

UniProt Primary Accession #

P02533

UniProt Secondary Accession #

Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4

UniProt Related Accession #

P02533

GenBank Nucleotide #

NM_000526.4

NCBI Official Full Name

keratin, type I cytoskeletal 14

NCBI Official Synonym Full Names

keratin 14, type I

NCBI Official Symbol

KRT14

NCBI Official Synonym Symbols

K14; NFJ; CK14; EBS3; EBS4

UniProt Protein Name

Keratin, type I cytoskeletal 14

UniProt Synonym Protein Names

Cytokeratin-14; CK-14; Keratin-14; K14

Protein Family

Keratin

UniProt Gene Name

KRT14

UniProt Synonym Gene Names

CK-14; K14

UniProt Entry Name

K1C14_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)

NCBI Summary for KRT14

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT14

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: keratin filament; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development; epithelial cell differentiation; response to zinc ion; intermediate filament bundle assembly; response to ionizing radiation; hair cycle; aging

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type

Research Articles on KRT14

1. plectin interacts with keratins 5 and 14 in a process associated with epidermolysis bullosa simplex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KRT14 recombinant protein

Reference Product	PubMed Publications
KRT5 recombinant protein	>93 publications with KRT14 and KRT5
EGFR recombinant protein	>7 publications with KRT14 and EGFR
DST recombinant protein	>3 publications with KRT14 and DST
IVL recombinant protein	>1 publications with KRT14 and IVL
CREBBP recombinant protein	>1 publications with KRT14 and CREBBP

Pathways associated with KRT14 recombinant protein

Products by Pathway	Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with KRT14 recombinant protein

Disease Name	Pubmed Publications
Epidermolysis Bullosa Simplex Antibodies	>94 publications with KRT14 and Epidermolysis Bullosa Simplex
Carcinoma Antibodies	>60 publications with KRT14 and Carcinoma
Carcinoma, Squamous Cell Antibodies	>38 publications with KRT14 and Carcinoma, Squamous Cell
Disease Models, Animal Antibodies	>24 publications with KRT14 and Disease Models, Animal
Hyperplasia Antibodies	>21 publications with KRT14 and Hyperplasia
Breast Neoplasms Antibodies	>18 publications with KRT14 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>13 publications with KRT14 and Neoplasms, Experimental
Necrosis Antibodies	>13 publications with KRT14 and Necrosis
Cell Transformation, Neoplastic Antibodies	>13 publications with KRT14 and Cell Transformation, Neoplastic
Inflammation Antibodies	>12 publications with KRT14 and Inflammation

Organs/Tissues associated with KRT14 recombinant protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>153 publications with KRT14 and Skin
Blood Antibodies	>25 publications with KRT14 and Blood
Mouth Antibodies	>16 publications with KRT14 and Mouth
Lung Antibodies	>10 publications with KRT14 and Lung
Eye Antibodies	>8 publications with KRT14 and Eye
Esophagus Antibodies	>8 publications with KRT14 and Esophagus
Mammary Gland Antibodies	>7 publications with KRT14 and Mammary Gland
Connective Tissue Antibodies	>6 publications with KRT14 and Connective Tissue
Liver Antibodies	>6 publications with KRT14 and Liver
Cervix Antibodies	>5 publications with KRT14 and Cervix