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Modification Methylase Bglii (bglIIM) Bacillus Subtilis Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1429331

This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease. More details

$575 / 0.02 mg (Baculovirus) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Modification methylase BglII (bglIIM), Recombinant Protein
Full Product Name
Recombinant Bacillus subtilis Modification methylase BglII
Product Synonym Names
N(4)- cytosine-specific methyltransferase BglII
Sequence
MSEDQYKQIK LHLGMEDDNE DLPNHIPSSF PKQHLNKIYN GDTMNMLLDI PDNSVDLVVT SPPYNINKFK NDRRPLEEYL KWQTEIIEQC HRVLKPSGSI FWQVGTYVND SGAHIPLDIR FFPIFESLGM FPRNRIVWVR PHGLHANKKF AGRHETILWF TKTPEYKFFL DPIRVPQKYA NKKHYKGDKK GELSGDPLGK NPGDVWAFRN VRHNHEEDTI HPTQYPEDMI ERIVLSTTEP NDIVLDPFIG MGTTASVAKN LNRYFYGAEI EKEYVDIAYQ ILSGEPDENN NFPNLKTLRQ YCEKNGIIDP SQYTFTRQRK GSKPSLDSKA HPEEHHKKEI VERIEFEAEN SVYKKVQNEQ
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
58 kDa
Sequence Positions
1-360aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q45489
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for bglIIM recombinant protein

Cloning and characterization of the BglII restriction-modification system reveals a possible evolutionary footprint.Anton B.P., Heiter D.F., Benner J.S., Hess E.J., Greenough L., Moran L.S., Slatko B.E., Brooks J.E.Gene 187:19-27(1997)

Other Notes

Small volumes of bglIIM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for bglIIM purchase

MBS1429331 is a recombinant protein targeting bglIIM. Also known as Recombinant Bacillus subtilis Modification methylase BglII. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for bglIIM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Modification methylase BglII
UniProt Protein Name
Modification methylase BglII
UniProt Synonym Protein Names
N(4)- cytosine-specific methyltransferase BglII
UniProt Gene Name
bglIIM
UniProt Synonym Gene Names
M.BglII
UniProt Entry Name
MTB2_BACIU

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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