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Vimentin (VIM) Bovine Native Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143077

Description: Bovine Vimentin protein produced in Bovine Lens, having a molecular weight of 57 kDa.Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin… More details

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Product Overview

Product Name
Vimentin, Native Protein
Full Product Name
Bovine Vimentin
Product Gene Name
VIM native protein
Product Synonym Names
Vimentin Bovine; Bovine Vimentin; Vimentin; Vim; FLJ36605; bVimentin
Host
Bovine Lens
Form/Format
The protein (1mg/ml) was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by SDS-PAGE.
Preparation and Storage
Lyophilized Bovine Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BovineVimentin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
53,728 Da
3D Structure
ModBase 3D Structure for P48616
Solubility
It is recommended to reconstitute the Bovine Vimentin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Reconstitution
After vimentin is dissolved in 6M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Sodium Phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco''s PBS).- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841.- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.

Product Categories/Family for VIM native protein

Recombinant & Natural Proteins; Recombinant Proteins; Vimentin

Other Notes

Small volumes of VIM native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS143077 is a native protein targeting VIM. Also known as Bovine Vimentin. Host: Bovine Lens.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
110347570
NCBI GeneID
280955
NCBI Accession #
NP_776394.2
UniProt Primary Accession #
P48616
UniProt Secondary Accession #
Q17QM7
UniProt Related Accession #
P48616
GenBank Nucleotide #
NM_173969.3
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_BOVIN
NCBI Protein Information
vimentin

UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; in utero embryonic development; intermediate filament organization; astrocyte development
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Research Articles on VIM

1. Immunocytochemistry for Vimentin detection in nuclei of IVF and NT bovine embryos

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with VIM native protein

 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram

Diseases associated with VIM native protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >420 publications with VIM and Nervous System Diseases
 Neurodegenerative Diseases Antibodies  >178 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >56 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >40 publications with VIM and Kidney Diseases
 Pain Antibodies  >37 publications with VIM and Pain
 Disease Models, Animal Antibodies  >36 publications with VIM and Disease Models, Animal
 Hemorrhage Antibodies  >34 publications with VIM and Hemorrhage
 Neoplasm Metastasis Antibodies  >33 publications with VIM and Neoplasm Metastasis
 Liver Diseases Antibodies  >33 publications with VIM and Liver Diseases
 Breast Neoplasms Antibodies  >31 publications with VIM and Breast Neoplasms

Organs/Tissues associated with VIM native protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >505 publications with VIM and Brain
 Blood Antibodies  >216 publications with VIM and Blood
 Muscle Antibodies  >118 publications with VIM and Muscle
 Kidney Antibodies  >60 publications with VIM and Kidney
 Liver Antibodies  >57 publications with VIM and Liver
 Lung Antibodies  >40 publications with VIM and Lung
 Embryonic Tissue Antibodies  >35 publications with VIM and Embryonic Tissue
 Skin Antibodies  >32 publications with VIM and Skin
 Reticulum Antibodies  >16 publications with VIM and Reticulum
 Intestine Antibodies  >15 publications with VIM and Intestine
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