Mouse mCD14 Recombinant Protein – Cat# MBS143088

CD14 (mCD14) Mouse Recombinant Protein

Name: mCD14 Recombinant Protein; CD14; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS143088
Price: 375 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143088

Description: The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by… More details

$375 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD14 (mCD14), Recombinant Protein

Full Product Name

Recombinant Mouse CD14

Product Gene Name

mCD14 recombinant protein

Product Synonym Names

CD14 Mouse; CD14 Mouse Recombinant; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14

Host

Chinese Hamster Ovarian Cells (CHO)

Form/Format

CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Sterile Filtered White lyophilized (freeze-dried) powder.

Preparation and Storage

Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse CD14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

39,204 Da

3D Structure

ModBase 3D Structure for P10810

Solubility

It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for mCD14 recombinant protein

Recombinant & Natural Proteins; CD Antigens

Applications Tested/Suitable for mCD14 recombinant protein

ELISA (EIA), Inhibition Assays, Western Blot (WB)

Other Notes

Small volumes of mCD14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mCD14 purchase

MBS143088 is a recombinant protein targeting mCD14. Also known as Recombinant Mouse CD14. Host: Chinese Hamster Ovarian Cells (CHO).

Additional Details

NCBI/Uniprot data below describe general gene information for mCD14. It may not necessarily be applicable to this product.

NCBI GI #

6753332

NCBI GeneID

12475

NCBI Accession #

NP_033971.1

UniProt Primary Accession #

P10810

UniProt Related Accession #

P10810

GenBank Nucleotide #

NM_009841.4

NCBI Official Full Name

monocyte differentiation antigen CD14

NCBI Official Synonym Full Names

CD14 antigen

NCBI Official Symbol

Cd14

UniProt Protein Name

Monocyte differentiation antigen CD14

UniProt Synonym Protein Names

Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14

Protein Family

Monocyte differentiation antigen

UniProt Gene Name

Cd14

UniProt Entry Name

CD14_MOUSE

NCBI Protein Information

monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

NCBI Summary for mCD14

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

UniProt Comments for mCD14

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion

Research Articles on mCD14

1. Data show that ingestion of sCD14 resulted in blood sCD14 levels up.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with mCD14 recombinant protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies	Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
IKK Complex Recruitment Mediated By RIP1 Pathway antibodies	IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Legionellosis Pathway antibodies	Legionellosis Pathway Diagram

Diseases associated with mCD14 recombinant protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>2321 publications with mCD14 and Inflammation
Necrosis Antibodies	>2311 publications with mCD14 and Necrosis
Cardiovascular Diseases Antibodies	>649 publications with mCD14 and Cardiovascular Diseases
Lung Diseases Antibodies	>531 publications with mCD14 and Lung Diseases
Liver Diseases Antibodies	>360 publications with mCD14 and Liver Diseases
Nervous System Diseases Antibodies	>359 publications with mCD14 and Nervous System Diseases
Disease Models, Animal Antibodies	>330 publications with mCD14 and Disease Models, Animal
Atherosclerosis Antibodies	>311 publications with mCD14 and Atherosclerosis
Heart Diseases Antibodies	>280 publications with mCD14 and Heart Diseases
Asthma Antibodies	>259 publications with mCD14 and Asthma

Organs/Tissues associated with mCD14 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7527 publications with mCD14 and Blood
Bone Antibodies	>1329 publications with mCD14 and Bone
Bone Marrow Antibodies	>1069 publications with mCD14 and Bone Marrow
Liver Antibodies	>624 publications with mCD14 and Liver
Lung Antibodies	>597 publications with mCD14 and Lung
Skin Antibodies	>407 publications with mCD14 and Skin
Kidney Antibodies	>334 publications with mCD14 and Kidney
Brain Antibodies	>307 publications with mCD14 and Brain
Muscle Antibodies	>281 publications with mCD14 and Muscle
Joint Antibodies	>279 publications with mCD14 and Joint