Gilthead Seabream Growth Hormone (sGH) Active Protein

Name: sGH Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS143189
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143189

Description: Somatotropin Gilthead Seabream Recombinant Sparus Aurata produced in E Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21.4 kDa. The Gilthead… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Gilthead Seabream Growth Hormone (sGH), Active Protein

Full Product Name

Recombinant Gilthead Seabream Growth Hormone

Product Gene Name

sGH active protein

Product Synonym Names

GH Gilthead Seabream; Growth Hormone Gilthead Seabream Recombinant; GH1; GH; GHN; GH-N; hGH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin

Sequence

The sequence of the first ten N-terminal amino acids of recombinan t Gilthead Seabream growth hormone was determined and was found to be Ala-Gln-Pr o-Ile-Thr- Asp-Gly-Gl n-Arg-Leu

Host

E Coli

Form/Format

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Growth-Hormone Gilthead Seabream although stable at room temperature for at least two weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization GH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Molecular Weight

23,105 Da

3D Structure

ModBase 3D Structure for P29971

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Growth-Hormone Gilthead Seabream in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

Biological Activity

Binding assays of the 125I-labeled gilthead seabream GH to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant gilthead seabream Growth Hormone, like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to Sparus aurata larvae or intraperitoneally to juvenile fish.

Product Categories/Family for sGH active protein

Cytokines and Growth Factors; Growth Factors; Growth Hormone

Other Notes

Small volumes of sGH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for sGH purchase

MBS143189 is an active protein targeting sGH. Also known as Recombinant Gilthead Seabream Growth Hormone. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for sGH. It may not necessarily be applicable to this product.

NCBI GI #

21903463

NCBI Accession #

P29971.3

UniProt Primary Accession #

P29971

UniProt Secondary Accession #

Q90Y60; Q91440

NCBI Official Full Name

Somatotropin

UniProt Protein Name

Somatotropin

UniProt Synonym Protein Names

Growth hormone

UniProt Gene Name

UniProt Entry Name

SOMA_SPAAU

UniProt Comments for sGH

Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.