Chicken chGH Active Protein – Cat# MBS143196

Growth Hormone (chGH) Chicken Active Protein

Name: chGH Active Protein; Growth Hormone; Chicken; MyBioSource
Brand: MyBioSource
SKU: MBS143196
Price: 470 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143196

Description: Somatotropin Chicken Antagonist Recombinant mutein G119R produced in E Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids with an additional Ala at the N-terminus and having a molecular mass of 22.3 kDa. The Chicken… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Growth Hormone (chGH), Active Protein

Full Product Name

Recombinant Chicken Growth Hormone Anatagonist

Product Gene Name

chGH active protein

Product Synonym Names

GH Antagonist Chicken; Growth Hormone Antagonist Chicken Recombinant; GH1; GH; GHN; GH-N; hGH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin; chGH Antagonist

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Ph e-Pro-Ala

Host

E Coli

Form/Format

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 pH-8.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Growth-Hormone Chicken antagonist although stable at room temperature for at least two weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization GH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Molecular Weight

23,110 Da

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Growth-Hormone Chicken antagonist in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

Protein Content

Protein quantitation was carried out by: UV spectroscopy at 280 nm using the absorbency value of 0.8 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Biological Activity

Recombinant Chicken Growth Hormone G119R mutant did not bind to ovine GHR-ECD and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine GH and chicken GH showed similar IC50 values in competition with 125I-ovine GH, while the IC50 of G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chGH G119R in homologous system.

Product Categories/Family for chGH active protein

Cytokines and Growth Factors; Growth Factors; Growth Hormone

Other Notes

Small volumes of chGH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for chGH purchase

MBS143196 is an active protein targeting chGH. Also known as Recombinant Chicken Growth Hormone Anatagonist. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for chGH. It may not necessarily be applicable to this product.

NCBI GI #

213890

NCBI Accession #

AAA49626.1

NCBI Official Full Name

growth hormone

UniProt Protein Name

Somatotropin

UniProt Synonym Protein Names

Growth hormone

Protein Family

Growth hormone

UniProt Gene Name

UniProt Entry Name

SOMA_ORENI

UniProt Comments for chGH

Growth hormone plays an important role in growth control and involved in the regulation of several anabolic processes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.