Human PEDF Recombinant Protein – Cat# MBS143336

Pigment Epithelium-Derived Factor (PEDF) Human Recombinant Protein

Name: PEDF Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS143336
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143336

PEDF Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5 kDa. The Human PEDF is purified by proprietary chromatographic techniques. More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Pigment Epithelium-Derived Factor (PEDF), Recombinant Protein

Full Product Name

Recombinant Human Pigment Epithelium-Derived Factor

Product Gene Name

PEDF recombinant protein

Product Synonym Names

PEDF Human; Pigment Epithelium-Derived Factor Human Recombinant; Pigment epithelium-derived factor; PEDF; Serpin-F1; SerpinF1; EPC-1; EPC1; PIG35

Sequence

MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP

Host

E Coli

Form/Format

The sterile filtered concentrated (1mg/ml) protein solution was lyophilized with 20mM sodium phosphate buffer & 150mM NaCl pH-7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95% as determined by SDS-PAGE and HPLC.

Preparation and Storage

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/ thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time.

OMIM

172860

3D Structure

ModBase 3D Structure for P36955

Solubility

It is recommended to reconstitute the lyophilized PEDF in sterile 18Ω-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for PEDF recombinant protein

Cytokines and Growth Factors; Neurotrophins; Pigment Epithelium-Derived Factor

Other Notes

Small volumes of PEDF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEDF purchase

MBS143336 is a recombinant protein targeting PEDF. Also known as Recombinant Human Pigment Epithelium-Derived Factor. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PEDF. It may not necessarily be applicable to this product.

NCBI GI #

39725934

NCBI GeneID

5176

NCBI Accession #

NP_002606.3

UniProt Primary Accession #

P36955

UniProt Secondary Accession #

Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092

UniProt Related Accession #

P36955

GenBank Nucleotide #

NM_002615.5

NCBI Official Full Name

pigment epithelium-derived factor

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

NCBI Official Symbol

SERPINF1

NCBI Official Synonym Symbols

OI6; OI12; PEDF; EPC-1; PIG35

UniProt Protein Name

Pigment epithelium-derived factor

UniProt Synonym Protein Names

Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

Protein Family

Pigment epithelium-derived factor

UniProt Gene Name

SERPINF1

UniProt Synonym Gene Names

PEDF; PEDF

UniProt Entry Name

PEDF_HUMAN

NCBI Protein Information

pigment epithelium-derived factor; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin F1

NCBI Summary for PEDF

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

UniProt Comments for PEDF

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; short-term memory; retina development in camera-type eye; negative regulation of inflammatory response; multicellular organismal development; response to arsenic; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi

Research Articles on PEDF

1. PEDF is anti-angiogenic and is associated with the growth inhibition of hemangioma-derived endothelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PEDF recombinant protein

Reference Product	PubMed Publications
VEGFA recombinant protein	>2 publications with PEDF and VEGFA
YWHAE recombinant protein	>1 publications with PEDF and YWHAE
A2M recombinant protein	>1 publications with PEDF and A2M

Diseases associated with PEDF recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>3 publications with PEDF and Nervous System Diseases
Proteinuria Antibodies	>1 publications with PEDF and Proteinuria
Brain Diseases Antibodies	>1 publications with PEDF and Brain Diseases
Urinary Bladder Neoplasms Antibodies	>1 publications with PEDF and Urinary Bladder Neoplasms
Lung Neoplasms Antibodies	>1 publications with PEDF and Lung Neoplasms
Cell Transformation, Neoplastic Antibodies	>1 publications with PEDF and Cell Transformation, Neoplastic
Weight Loss Antibodies	>1 publications with PEDF and Weight Loss

Organs/Tissues associated with PEDF recombinant protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>22 publications with PEDF and Bone
Nerve Antibodies	>21 publications with PEDF and Nerve
Eye Antibodies	>19 publications with PEDF and Eye
Connective Tissue Antibodies	>9 publications with PEDF and Connective Tissue
Blood Antibodies	>6 publications with PEDF and Blood
Skin Antibodies	>5 publications with PEDF and Skin
Adipose Tissue Antibodies	>5 publications with PEDF and Adipose Tissue
Brain Antibodies	>5 publications with PEDF and Brain
Embryonic Tissue Antibodies	>3 publications with PEDF and Embryonic Tissue
Lung Antibodies	>2 publications with PEDF and Lung