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Deoxyribodipyrimidine Photo-Lyase (phrB) E Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143367

Description: 800x600 800x600 phrB E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 495 amino acids (1-472) and having a molecular mass of 56.1kDa.phrB is fused to a 23 amino acid His-tag at N-terminus & purified… More details

$250 / 0.002 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Deoxyribodipyrimidine photo-lyase (phrB), Recombinant Protein
Full Product Name
Recombinant E Coli Deoxyribodipyrimidine photo-lyase
Product Gene Name
phrB recombinant protein
Product Synonym Names
phrB E.Coli; Deoxyribodipyrimidine photo-lyase E.Coli Recombinant; 800x600 ECK0697; JW0698; phrB; phr; Deoxyribodipyrimidine photo-lyase; DNA photolyase; Photoreactivating enzyme; EC=4.1.99.3; b0708. 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Sequence
MGSSHHH HHH SSGLVPRGSH MGSMTT HLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK
Host
E Coli
Form/Format
800x600 The phrB solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 1mM DTT. Normal 0 false false false EN-US X-NONE HE MicrosoftInternetExplorer4 DefSemiHidden="true" DefQFormat="false" DefPriority="99" LatentStyleCo
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Molecular Weight
53,667 Da
3D Structure
ModBase 3D Structure for P00914

Product Categories/Family for phrB recombinant protein

Enzymes

Other Notes

Small volumes of phrB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for phrB purchase

MBS143367 is a recombinant protein targeting phrB. Also known as Recombinant E Coli Deoxyribodipyrimidine photo-lyase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for phrB. It may not necessarily be applicable to this product.

NCBI GI #
16128683
NCBI GeneID
947005
NCBI Accession #
NP_415236.1
UniProt Primary Accession #
P00914
GenBank Nucleotide #
NC_000913.3
NCBI Official Full Name
deoxyribodipyrimidine photolyase, FAD-binding
NCBI Official Symbol
phr
NCBI Official Synonym Symbols
ECK0697; JW0698; phrB
UniProt Protein Name
Deoxyribodipyrimidine photo-lyase
UniProt Synonym Protein Names
DNA photolyase; Photoreactivating enzyme
Protein Family
(6-4) photolyase
UniProt Gene Name
phrB
UniProt Synonym Gene Names
phr
UniProt Entry Name
PHR_ECOLI
NCBI Protein Information
deoxyribodipyrimidine photolyase, FAD-binding

NCBI Summary for phrB

The flavoprotein DNA photolyase uses light to repair cyclobutylpyrimidine dimers in DNA. [More information is available at EcoGene: EG10736]. Exposing Escherichia coli to UV radiation at wavelengths near the absorption maximum of DNA produces cyclobutane-type pyrimidine dimers (CPDs) as well as, to a lesser degree, 6-4 pyrimidine-pyrimidones in its DNA. [More information is available at EcoCyc: EG10736].
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UniProt Comments for phrB

Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

Research Articles on phrB

1. Data show that DNA photolyase exhibits a distinct reduction-potential gradient along the same aromatic residues with favorable reorganization energies to drive a highly unidirectional electron flow toward the active-site center from the protein surface.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with phrB recombinant protein

 Reference Product  PubMed Publications
 uvrB recombinant protein  >9 publications with phrB and uvrB
 uvrA recombinant protein  >9 publications with phrB and uvrA
 uvrC recombinant protein  >3 publications with phrB and uvrC
 fadH recombinant protein  >1 publications with phrB and fadH
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