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[Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 1, Mitochondrial (PDK1) Rat Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1434227

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity… More details

$1145 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (Pdk1), Recombinant Protein
Full Product Name
Recombinant Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (Pdk1)
Product Gene Name
Product Synonym Gene Name
Pdk1
Sequence
ASDSASGSGP ASESGVPGQV DFYARFSPSP LSMKQFLDFG SVNACEKTSF MFLRQELPVR LANIMKEISL LPDNLLRTPS VQLVQSWYIQ SLQELLDFKD KSAEDAKTIY EFTDTVIRIR NRHNDVIPTM AQGVTEYKES FGVDPVTSQN VQYFLDRFYM SRISIRMLLN QHSLLFGGKG SPSHRKHIGS INPNCDVVEV IKDGYENARR LCDLYYVNSP ELELEELNAK SPGQPIQVVY VPSHLYHMVF ELFKNAMRAT MEHHADKGVY PPIQVHVTLG EEDLTVKMSD RGGGVPLRKI DRLFNYMYST APRPRVETSR AVPLAGFGYG LPISRLYAQY FQGDLKLYSL EGYGTDAVIY IKALSTESIE RLPVYNKAAW KHYRTNHEAD DWCVPSREPK DMTTFRSS
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
49,081 Da
Sequence Positions
27-434, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q63065
Species
Rat
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of Pdk1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pdk1 purchase

MBS1434227 is a recombinant protein targeting Pdk1. Also known as Recombinant Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (Pdk1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Pdk1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase kinase 1
NCBI Official Symbol
Pdk1
UniProt Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
UniProt Synonym Protein Names
PDK p48; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1
UniProt Gene Name
Pdk1
UniProt Synonym Gene Names
Pdh; PDH kinase 1
NCBI Protein Information
pyruvate dehydrogenase kinase, isozyme 1

NCBI Summary for Pdk1

catalyzes the phosphorylation and inactivation of the pyruvate dehydrogenase complex [RGD, Feb 2006]

UniProt Comments for Pdk1

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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