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Modified Affinity Purified Avidin (AVD) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143462

Description: Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and… More details

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Technical Datasheet View PDF
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Product Overview

Product Name
Modified Affinity Purified Avidin (AVD), Active Protein
Full Product Name
Modified Affinity Purified Avidin
Product Gene Name
Product Synonym Names
Avidin Biotin; Modified Affinity Purified Avidin; Avidin; AVD; AVID; Avidin-Biotin
Form/Format
Salt-free lyophilized powder with no preservative added.
Lyophilized freeze dry Powder.
Preparation and Storage
Store protein solution at 2-8 degree C.
Molecular Weight
16,768 Da
3D Structure
ModBase 3D Structure for P02701
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Advantages
1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin. 2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin. 3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.
Solubility
We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.
Activity
Binding activity of Avidin-Biotin is 10-15units/mg protein. One unit of Avidin activity is defined as the amount of protein which will bind 1 ug d-biotin.

Other Notes

Small volumes of AVD active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AVD purchase

MBS143462 is an active protein targeting AVD. Also known as Modified Affinity Purified Avidin.

Additional Details

NCBI/Uniprot data below describe general gene information for AVD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
avidin
NCBI Official Synonym Full Names
avidin
NCBI Official Symbol
AVD
UniProt Protein Name
Avidin
Protein Family
UniProt Gene Name
AVD
UniProt Entry Name
AVID_CHICK
NCBI Protein Information
avidin; mature avidin

UniProt Comments for AVD

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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