Human MIP 3 Active Protein – Cat# MBS143488

Macrophage Inflammatory Protein-3 (MIP 3) Human Active Protein

Name: MIP 3 Active Protein; Macrophage Inflammatory Protein-3; Human; MyBioSource
Brand: MyBioSource
SKU: MBS143488
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143488

Description: MIP-3 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa. The MIP-3 is purified by proprietary chromatographic techniques.Introduction: CCL23… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Macrophage Inflammatory protein-3 (MIP 3), Active Protein

Full Product Name

Recombinant Human Macrophage Inflammatory protein-3 (CCL23)

Product Gene Name

MIP 3 active protein

Product Synonym Names

MIP 3 Human; Macrophage Inflammatory Protein-3 Human Recombinant (CCL23); C-C motif chemokine 23; Small-inducible cytokine A23; Macrophage inflammatory protein 3; Myeloid progenitor inhibitory factor 1; CK-beta-8; MIP-3; MPIF-1; CKB-8; CCL23; MIP3; MPIF1; SCYA23; CKb8; Ckb-8-1

Sequence

RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN

Host

E Coli

Form/Format

Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized MIP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL23 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

15,290 Da

OMIM

602494

3D Structure

ModBase 3D Structure for P55773

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized MIP-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

Determined by its ability to chemoattract human T cell population using a concentration range of 10-50ng/ml corresponding to a Specific Activity of 20,000-100,000IU/mg.

Product Categories/Family for MIP 3 active protein

Chemokines; MIP

Other Notes

Small volumes of MIP 3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP 3 purchase

MBS143488 is an active protein targeting MIP 3. Also known as Recombinant Human Macrophage Inflammatory protein-3 (CCL23). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MIP 3. It may not necessarily be applicable to this product.

NCBI GI #

632794829

NCBI GeneID

6368

NCBI Accession #

NP_005055.3

UniProt Primary Accession #

P55773

UniProt Secondary Accession #

O00174; O75950; Q52LD4; B7ZKQ3

UniProt Related Accession #

P55773

GenBank Nucleotide #

NM_005064.5

NCBI Official Full Name

C-C motif chemokine 23 isoform CKbeta8-1

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 23

NCBI Official Symbol

CCL23

NCBI Official Synonym Symbols

CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8

UniProt Protein Name

C-C motif chemokine 23

UniProt Synonym Protein Names

CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; Myeloid progenitor inhibitory factor 1; MPIF-1; Small-inducible cytokine A23Cleaved into the following 4 chains:CCL23(19-99); CCL23(22-99); CCL23(27-99); CCL23(30-99)

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL23

UniProt Synonym Gene Names

MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1

UniProt Entry Name

CCL23_HUMAN

NCBI Protein Information

C-C motif chemokine 23; C6 beta-chemokine; macrophage inflammatory protein 3; myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23

NCBI Summary for MIP 3

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

UniProt Comments for MIP 3

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cell-cell signaling; immune response; chemotaxis; inflammatory response; signal transduction

Research Articles on MIP 3

1. ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIP 3 active protein

Reference Product	PubMed Publications
CCR1 active protein	>17 publications with MIP 3 and CCR1
CCR2 active protein	>5 publications with MIP 3 and CCR2
CCR3 active protein	>4 publications with MIP 3 and CCR3
CXCR4 active protein	>3 publications with MIP 3 and CXCR4
CCR5 active protein	>3 publications with MIP 3 and CCR5
CX3CR1 active protein	>2 publications with MIP 3 and CX3CR1
CXCR3 active protein	>1 publications with MIP 3 and CXCR3

Pathways associated with MIP 3 active protein

Products by Pathway	Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies	Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram

Diseases associated with MIP 3 active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>8 publications with MIP 3 and Necrosis
Carcinoma Antibodies	>7 publications with MIP 3 and Carcinoma
Cardiovascular Diseases Antibodies	>6 publications with MIP 3 and Cardiovascular Diseases
Colorectal Neoplasms Antibodies	>4 publications with MIP 3 and Colorectal Neoplasms
Atherosclerosis Antibodies	>4 publications with MIP 3 and Atherosclerosis
Leukemia, Myeloid, Acute Antibodies	>3 publications with MIP 3 and Leukemia, Myeloid, Acute
Heart Diseases Antibodies	>2 publications with MIP 3 and Heart Diseases
Fever Antibodies	>2 publications with MIP 3 and Fever
Fibrosis Antibodies	>1 publications with MIP 3 and Fibrosis
Atrophy Antibodies	>1 publications with MIP 3 and Atrophy

Organs/Tissues associated with MIP 3 active protein

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>2 publications with MIP 3 and Placenta
Liver Antibodies	>2 publications with MIP 3 and Liver
Lung Antibodies	>1 publications with MIP 3 and Lung
Uncharacterized Tissue Antibodies	>1 publications with MIP 3 and Uncharacterized Tissue