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Lactate Dehydrogenase A (LDHA) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143494

Description: LDHA Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 38.8 kDa. The LDHA is fused to a 20 amino acid his tag at N-terminus and purified by… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Lactate Dehydrogenase A (LDHA), Active Protein
Full Product Name
Recombinant Human Lactate Dehydrogenase A
Product Gene Name
Product Synonym Names
LDHA Human; Lactate Dehydrogenase A Human Recombinant; LDH-A; GSD11; LDH1; LDHM; PIG19; EC 1.1.1.27; lactate dehydrogenase M; LDH-M; LDH-1; L-lactate dehydrogenase A chain; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; Cell proliferation-inducing gene 19 protein; LDHA
Sequence
MGSSHHH HHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
Host
E Coli
Form/Format
The LDHA protein solution contains 20mM Tris-HCl pH-8, 100mM NaCl and 20% glycerol.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
26,712 Da
OMIM
150000
3D Structure
ModBase 3D Structure for P00338
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37 degree C.

Product Categories/Family for LDHA active protein

Other Notes

Small volumes of LDHA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHA purchase

MBS143494 is an active protein targeting LDHA. Also known as Recombinant Human Lactate Dehydrogenase A. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase A chain isoform 2
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
LDHA
NCBI Official Synonym Symbols
LDH1; LDHM; GSD11; PIG19; HEL-S-133P
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
UniProt Entry Name
LDHA_HUMAN
NCBI Protein Information
L-lactate dehydrogenase A chain; LDH muscle subunit; LDH-A; LDH-M; cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; lactate dehydrogenase M; renal carcinoma antigen NY-REN-59

NCBI Summary for LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for LDHA

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Amino Acid Metabolism - cysteine and methionine; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.27; Carbohydrate Metabolism - propanoate; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; L-lactate dehydrogenase activity

Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process

Disease: Glycogen Storage Disease Xi
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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