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Leptin Triple Antagonist Ovine Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143655

Description: Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 35.6 kDa, Leptin was mutated, resulting in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Leptin Triple Antagonist, Active Protein
Full Product Name
Recombinant Ovine Leptin Triple Antagonist, Pegylated
Product Synonym Names
Leptin tA Ovine, PEG; Leptin Antagonist Triple Mutant Ovine Recombinant, Pegylated; oLeptin tA PEG
Host
E Coli
Form/Format
The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 Lep-tA mutant mg/ml and up to 2mM and filter sterilization Leptin mutant can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Biological Activity
Pegylated triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist like in mouse leptin antagonists), resulting mainly from increased food intake.

Product Categories/Family for Leptin Triple Antagonist active protein

Cytokines and Growth Factors; Growth Factors; Leptin

Other Notes

Small volumes of Leptin Triple Antagonist active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Leptin Triple Antagonist purchase

MBS143655 is an active protein targeting Leptin Triple Antagonist. Also known as Recombinant Ovine Leptin Triple Antagonist, Pegylated. Host: E Coli.
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