Runt-Related Transcription Factor 3 (RUNX3) Human Recombinant Protein

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; nucleolus; nucleus

Molecular Function: protein binding; transcription factor activity; ATP binding

Biological Process: peripheral nervous system neuron development; transcription from RNA polymerase II promoter; axon guidance; hair follicle morphogenesis; regulation of transcription, DNA-dependent; cell maturation; interferon-gamma production; chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; negative regulation of cell cycle; negative regulation of epithelial cell proliferation