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Enoyl Coa Hydratase 1 (ECH1) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144117

Description: ECH1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (34-328a.a.) and having a molecular mass of 34.4kDa.ECH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Enoyl CoA Hydratase 1 (ECH1), Recombinant Protein
Full Product Name
Recombinant Human Enoyl CoA Hydratase 1, Peroxisomal
Product Gene Name
Product Synonym Names
ECH1 Human; Enoyl CoA Hydratase 1, Peroxisomal Human Recombinant; peroxisomal; enoyl Coenzyme A hydratase 1
Sequence
MGSSHHH HHH SSGLVPRGSH MTGSSA QEAA SGVALGEAPD HSYESLRVTS AQKHVLHVQL NRPNKRNAMN KVFWREMVEC FNKISRDADC RAVVISGAGK MFTAGIDLMD MASDILQPKG DDVARISWYL RDIITRYQET FNVIERCPKP VIAAVHGGCI GGGVDLVTAC DIRYCAQDAF FQVKEVDVGL AADVGTLQRL PKVIGNQSLV NELAFTARKM MADEALGSGL VSRVFPDKEV MLDAALALAA EISSKSPVAV QSTKVNLLYS RDHSVAESLN YVASWNMSML QTQDLVKSVQ ATTENKELKT VTFSKL
Host
E Coli
Form/Format
The ECH1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 50mM NaCl, and 10% glycerol.
Sterile Filtered clear solution.
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
35,816 Da
OMIM
600696
3D Structure
ModBase 3D Structure for Q13011

Product Categories/Family for ECH1 recombinant protein

Other Notes

Small volumes of ECH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ECH1 purchase

MBS144117 is a recombinant protein targeting ECH1. Also known as Recombinant Human Enoyl CoA Hydratase 1, Peroxisomal. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ECH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
NCBI Official Synonym Full Names
enoyl CoA hydratase 1, peroxisomal
NCBI Official Symbol
ECH1
NCBI Official Synonym Symbols
HPXEL
UniProt Protein Name
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Protein Family
UniProt Gene Name
ECH1
UniProt Entry Name
ECH1_HUMAN
NCBI Protein Information
delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; peroxisomal enoyl-CoA hydratase 1

NCBI Summary for ECH1

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
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UniProt Comments for ECH1

ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Amino Acid Metabolism - tyrosine; Mitochondrial; Isomerase; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane; mitochondrion; peroxisome

Molecular Function: protein binding; isomerase activity; receptor binding

Biological Process: fatty acid beta-oxidation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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