Human GSS Recombinant Protein – Cat# MBS144170

Glutathione Synthetase (GSS) Human Recombinant Protein

Name: GSS Recombinant Protein; Glutathione Synthetase; Human; MyBioSource
Brand: MyBioSource
SKU: MBS144170
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144170

Description: GSS Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 494 amino acids (1-474 a.a.) and having a molecular mass of 54.5kDa. The GSS is purified by… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Glutathione Synthetase (GSS), Recombinant Protein

Full Product Name

Recombinant Human Glutathione Synthetase

Product Gene Name

GSS recombinant protein

Product Synonym Names

GSS Human; Glutathione Synthetase Human Recombinant; Glutathione synthetase; GSH synthetase; GSH-S; Glutathione synthase; GSHS; MGC14098; GSS

Sequence

MGSSHHH HQH SSGLVPRGSH MATNWGSLLQ DKQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF TLFPSLVPSA LLEQAYAVQM DFNLLVDAVS QNAAFLEQTL SSTIKQDDFT ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QRSADGSPAL KQIEINTISA SFGGLASRTP AVHRHVLSVL SKTKEAGKIL SNNPSKGLAL GIAKAWELYG SPNALVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRTFE DISEKGSLDQ DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSH AAKCPDIATQ LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDVGEEGDQA IAEALAAPSR FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK IEPEPFENCL LRPGSPARVV QCISELGIFG VYVRQEKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV

Host

E Coli

Form/Format

The GSS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.

Purity/Purification

Greater than 95.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

OMIM

231900

3D Structure

ModBase 3D Structure for P48637

Appearance

Sterile filtered colorless solution

Product Categories/Family for GSS recombinant protein

Enzymes; Synthetase

Other Notes

Small volumes of GSS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSS purchase

MBS144170 is a recombinant protein targeting GSS. Also known as Recombinant Human Glutathione Synthetase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GSS. It may not necessarily be applicable to this product.

NCBI GI #

4504169

NCBI GeneID

2937

NCBI Accession #

NP_000169.1

UniProt Primary Accession #

P48637

UniProt Secondary Accession #

Q4TTD9; B2R697; B6F210; E1P5P9

UniProt Related Accession #

P48637

GenBank Nucleotide #

NM_000178.2

NCBI Official Full Name

glutathione synthetase

NCBI Official Synonym Full Names

glutathione synthetase

NCBI Official Symbol

GSS

NCBI Official Synonym Symbols

GSHS; HEL-S-64p; HEL-S-88n

UniProt Protein Name

Glutathione synthetase

UniProt Synonym Protein Names

Glutathione synthase

Protein Family

Bifunctional glutathionylspermidine synthetase/amidase

UniProt Gene Name

GSS

UniProt Synonym Gene Names

GSH synthetase; GSH-S

UniProt Entry Name

GSHB_HUMAN

NCBI Protein Information

glutathione synthetase; GSH synthetase; GSH-S; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; glutathione synthase

NCBI Summary for GSS

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]

UniProt Comments for GSS

GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.

Protein type: Other Amino Acids Metabolism - glutathione; Ligase; EC 6.3.2.3

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding

Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging

Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To

Research Articles on GSS

1. Its depletion causes protein oxidization in ATL cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GSS recombinant protein

Reference Product	PubMed Publications
GCLC recombinant protein	>12 publications with GSS and GCLC
GPX1 recombinant protein	>8 publications with GSS and GPX1
GSR recombinant protein	>8 publications with GSS and GSR
GPX4 recombinant protein	>2 publications with GSS and GPX4
GSTA1 recombinant protein	>1 publications with GSS and GSTA1

Pathways associated with GSS recombinant protein

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway antibodies	Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway Diagram
Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway antibodies	Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway Diagram
Defective GGT1 Causes Glutathionuria (GLUTH) Pathway antibodies	Defective GGT1 Causes Glutathionuria (GLUTH) Pathway Diagram
Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway antibodies	Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway Diagram
Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway antibodies	Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway Diagram
Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway antibodies	Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway Diagram
Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway antibodies	Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway Diagram
Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway antibodies	Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway Diagram
Defective UGT1A1 Causes Hyperbilirubinemia Pathway antibodies	Defective UGT1A1 Causes Hyperbilirubinemia Pathway Diagram

Diseases associated with GSS recombinant protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>99 publications with GSS and Immune System Diseases
Disease Models, Animal Antibodies	>29 publications with GSS and Disease Models, Animal
Inflammation Antibodies	>25 publications with GSS and Inflammation
Cardiovascular Diseases Antibodies	>24 publications with GSS and Cardiovascular Diseases
Carcinoma Antibodies	>19 publications with GSS and Carcinoma
Chromosome Aberrations Antibodies	>17 publications with GSS and Chromosome Aberrations
Nerve Degeneration Antibodies	>13 publications with GSS and Nerve Degeneration
Lung Neoplasms Antibodies	>11 publications with GSS and Lung Neoplasms
Necrosis Antibodies	>10 publications with GSS and Necrosis
Heart Diseases Antibodies	>10 publications with GSS and Heart Diseases

Organs/Tissues associated with GSS recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>224 publications with GSS and Brain
Blood Antibodies	>107 publications with GSS and Blood
Nerve Antibodies	>44 publications with GSS and Nerve
Bone Antibodies	>38 publications with GSS and Bone
Skin Antibodies	>37 publications with GSS and Skin
Muscle Antibodies	>37 publications with GSS and Muscle
Vascular Antibodies	>33 publications with GSS and Vascular
Liver Antibodies	>32 publications with GSS and Liver
Eye Antibodies	>24 publications with GSS and Eye
Lung Antibodies	>21 publications with GSS and Lung