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Methyltransferase Like 1 (METTL1) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144233

Description: Recombinant Human METTL1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (1-276a.a.) and having a molecular mass of 33.6kDa.METTL1 is fused to a 20 amino acid His-tag at N-terminus & purified by… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Methyltransferase Like 1 (METTL1), Recombinant Protein
Full Product Name
Recombinant Human Methyltransferase Like 1
Product Synonym Names
METTL1 Human; Methyltransferase Like 1 Human Recombinant; Methyltransferase-Like 1; TRM8; tRNA(m7G46)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; C12orf1; YDL201w; D1075-like gene product; FLJ95748; EC 2.1.1.33
Sequence
MGSSHHH HHH SSGLVPRGSH MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH
Host
E Coli
Form/Format
The METTL1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT, 50mM NaCl and 10% glycerol.
Sterile Filtered clear solution.
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
17,423 Da
OMIM
604466
3D Structure
ModBase 3D Structure for Q9UBP6

Product Categories/Family for METTL1 recombinant protein

Other Notes

Small volumes of METTL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for METTL1 purchase

MBS144233 is a recombinant protein targeting METTL1. Also known as Recombinant Human Methyltransferase Like 1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for METTL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA (guanine-N(7)-)-methyltransferase isoform a
NCBI Official Synonym Full Names
methyltransferase like 1
NCBI Official Symbol
METTL1
NCBI Official Synonym Symbols
TRM8; TRMT8; C12orf1; YDL201w
UniProt Protein Name
tRNA (guanine-N(7)-)-methyltransferase
UniProt Synonym Protein Names
Methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
UniProt Gene Name
METTL1
UniProt Entry Name
TRMB_HUMAN
NCBI Protein Information
tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase

NCBI Summary for METTL1

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]
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UniProt Comments for METTL1

METTL1: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Belongs to the methyltransferase superfamily. TrmB family.

Protein type: Methyltransferase; EC 2.1.1.33; RNA processing

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; tRNA (guanine-N7-)-methyltransferase activity; tRNA binding

Biological Process: tRNA modification; tRNA methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with METTL1 recombinant protein

Organs/Tissues associated with METTL1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with METTL1 and Lung
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