Human HSPA5 Recombinant Protein – Cat# MBS144355

Heat Shock 70kDa Protein 5 (HSPA5) Human Recombinant Protein

Name: HSPA5 Recombinant Protein; Heat Shock 70kDa Protein 5; Human; MyBioSource
Brand: MyBioSource
SKU: MBS144355
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144355

Description: Recombinant Human HSPA5 produced in E Coli is a single,non-glycosylated polypeptide chain containing 640 amino acids (20-650 a.a.) and having a molecular mass of 71kDa. HSPA5 human recombinant is fused to an 8 amino acid His Tag at C-terminus and… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Heat Shock 70kDa protein 5 (HSPA5), Recombinant Protein

Full Product Name

Recombinant Human Heat Shock 70kDa protein 5

Product Gene Name

HSPA5 recombinant protein

Product Synonym Names

HSPA5 Human; Heat Shock 70kDa Protein 5 Human Recombinant; BIP; MIF2; GRP78; FLJ26106; HSPA5; 78 kDa glucose-regulated protein; GRP 78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

Sequence

MEEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AELEHHH HHH

Host

E Coli

Form/Format

The HSPA5 protein solution contains 20mM Tris-HCl, pH-8 & 10% Glycerol.
Sterile filtered colorless solution.

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Molecular Weight

72,333 Da

OMIM

138120

3D Structure

ModBase 3D Structure for P11021

Product Categories/Family for HSPA5 recombinant protein

Heat Shock Proteins; Recombinant Proteins; Heat Shock Protein

Other Notes

Small volumes of HSPA5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSPA5 purchase

MBS144355 is a recombinant protein targeting HSPA5. Also known as Recombinant Human Heat Shock 70kDa protein 5. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPA5. It may not necessarily be applicable to this product.

NCBI GI #

16507237

NCBI GeneID

3309

NCBI Accession #

NP_005338.1

UniProt Primary Accession #

P11021

UniProt Secondary Accession #

Q2EF78; Q9NPF1; Q9UK02; B0QZ61

UniProt Related Accession #

P11021

GenBank Nucleotide #

NM_005347.4

NCBI Official Full Name

78 kDa glucose-regulated protein

NCBI Official Synonym Full Names

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

NCBI Official Symbol

HSPA5

NCBI Official Synonym Symbols

BIP; MIF2; GRP78; HEL-S-89n

UniProt Protein Name

78 kDa glucose-regulated protein

UniProt Synonym Protein Names

Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

UniProt Gene Name

HSPA5

UniProt Synonym Gene Names

GRP78; GRP-78; BiP

UniProt Entry Name

GRP78_HUMAN

NCBI Protein Information

78 kDa glucose-regulated protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; immunoglobulin heavy chain-binding protein

NCBI Summary for HSPA5

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

UniProt Comments for HSPA5

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; focal adhesion; cell surface; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; substantia nigra development; platelet degranulation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; negative regulation of apoptosis; positive regulation of cell migration

Research Articles on HSPA5

1. oxidized phospholipids binding to plasma membrane-localized GRP78 leads to GRP78 trafficking to caveolin-enriched microdomains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HSPA5 recombinant protein

Reference Product	PubMed Publications
ATF4 recombinant protein	>32 publications with HSPA5 and ATF4
ATF6 recombinant protein	>31 publications with HSPA5 and ATF6
ERN1 recombinant protein	>8 publications with HSPA5 and ERN1
HYOU1 recombinant protein	>7 publications with HSPA5 and HYOU1
SIL1 recombinant protein	>5 publications with HSPA5 and SIL1
EIF2AK3 recombinant protein	>3 publications with HSPA5 and EIF2AK3

Pathways associated with HSPA5 recombinant protein

Products by Pathway	Pathway Diagram
ATF6-alpha Activates Chaperone Genes Pathway antibodies	ATF6-alpha Activates Chaperone Genes Pathway Diagram
ATF6-alpha Activates Chaperones Pathway antibodies	ATF6-alpha Activates Chaperones Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies	Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
IRE1alpha Activates Chaperones Pathway antibodies	IRE1alpha Activates Chaperones Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with HSPA5 recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>65 publications with HSPA5 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>65 publications with HSPA5 and Nervous System Diseases
Disease Models, Animal Antibodies	>59 publications with HSPA5 and Disease Models, Animal
Brain Diseases Antibodies	>55 publications with HSPA5 and Brain Diseases
Inflammation Antibodies	>35 publications with HSPA5 and Inflammation
Liver Diseases Antibodies	>23 publications with HSPA5 and Liver Diseases
Heart Diseases Antibodies	>22 publications with HSPA5 and Heart Diseases
Necrosis Antibodies	>22 publications with HSPA5 and Necrosis
Kidney Diseases Antibodies	>16 publications with HSPA5 and Kidney Diseases
Hyperglycemia Antibodies	>11 publications with HSPA5 and Hyperglycemia

Organs/Tissues associated with HSPA5 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>82 publications with HSPA5 and Brain
Blood Antibodies	>52 publications with HSPA5 and Blood
Muscle Antibodies	>50 publications with HSPA5 and Muscle
Heart Antibodies	>40 publications with HSPA5 and Heart
Liver Antibodies	>40 publications with HSPA5 and Liver
Kidney Antibodies	>34 publications with HSPA5 and Kidney
Vascular Antibodies	>23 publications with HSPA5 and Vascular
Nerve Antibodies	>20 publications with HSPA5 and Nerve
Pancreas Antibodies	>17 publications with HSPA5 and Pancreas
Lung Antibodies	>15 publications with HSPA5 and Lung