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Dihydrolipoamide S-Succinyltransferase (DLST) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144463

Description: DLST is a Full-length cDNA coding for the mature form of the human OGDC-E2 protein having a molecular mass of 42,301 Dalton (pH 6.3).DLST protein is fused to a hexa-histidine purification tag.Introduction: DLST catalyzes the general… More details

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Product Overview

Online Datasheet in PDF
Product Name
Dihydrolipoamide S-Succinyltransferase (DLST), Recombinant Protein
Full Product Name
Recombinant Human Dihydrolipoamide S-Succinyltransferase
Product Gene Name
DLST recombinant protein
Product Synonym Names
DLST Human; Dihydrolipoamide S-Succinyltransferase Human Recombinant; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; component E2; DLST; E2K; OGDC-E2; EC 2.3.1.61; EC 2.3.1
Host
Sf9 Insect Cells
Form/Format
DLST (0.74mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Purity/Purification
DLST purity was found to be greater than 75% as determined by SDS-PAGE.
Concentration
0.4-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (lot specific)
Molecular Weight
39,553 Da
OMIM
126063
3D Structure
ModBase 3D Structure for P36957
Immunological Functions
DLST binds IgG-type human auto-antibodies.

Product Categories/Family for DLST recombinant protein

Enzymes; Transferase
Applications Tested/Suitable for DLST recombinant protein
Western Blot (WB), ELISA (EIA), Immunodot
Application Notes for DLST recombinant protein
Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody.
Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.

Other Notes

Small volumes of DLST recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DLST purchase

MBS144463 is a recombinant protein targeting DLST. Also known as Recombinant Human Dihydrolipoamide S-Succinyltransferase. Host: Sf9 Insect Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for DLST. It may not necessarily be applicable to this product.

NCBI GI #
19923748
NCBI GeneID
1743
NCBI Accession #
NP_001924.2
UniProt Primary Accession #
P36957
UniProt Secondary Accession #
Q7LDY7; Q9BQ32; B7Z5W8; E7ESY5
UniProt Related Accession #
P36957
GenBank Nucleotide #
NM_001933.4
NCBI Official Full Name
dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
NCBI Official Symbol
DLST
NCBI Official Synonym Symbols
DLTS
UniProt Protein Name
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
UniProt Synonym Protein Names
2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; E2K
Protein Family
Probable serine transporter
UniProt Gene Name
DLST
UniProt Synonym Gene Names
DLTS; OGDC-E2
UniProt Entry Name
ODO2_HUMAN
NCBI Protein Information
dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; E2K; OGDC-E2

NCBI Summary for DLST

This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for DLST

DLST: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Forms a 24-polypeptide structural core with octahedral symmetry. Belongs to the 2-oxoacid dehydrogenase family.

Protein type: Mitochondrial; Transferase; EC 2.3.1.61; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: membrane; mitochondrial matrix; oxoglutarate dehydrogenase complex; nucleus

Molecular Function: dihydrolipoyllysine-residue succinyltransferase activity

Biological Process: cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process
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Research Articles on DLST

1. ATP consumption is demonstrated in respiration-impaired isolated and in situ neuronal somal mitochondria from transgenic mice that exhibit a 20-48% decrease in alpha-ketoglutarate dehydrogenase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DLST recombinant protein

 Reference Product  PubMed Publications
 DLD recombinant protein  >2 publications with DLST and DLD
 IDH3G recombinant protein  >1 publications with DLST and IDH3G
 IDH3A recombinant protein  >1 publications with DLST and IDH3A
 OGDH recombinant protein  >1 publications with DLST and OGDH

Pathways associated with DLST recombinant protein

 Products by Pathway  Pathway Diagram
 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway antibodies  2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway Diagram
 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway antibodies  2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram

Diseases associated with DLST recombinant protein

 Disease Name  Pubmed Publications
 Drug-Related Side Effects and Adverse Reactions Antibodies  >28 publications with DLST and Drug-Related Side Effects and Adverse Reactions
 Liver Diseases Antibodies  >13 publications with DLST and Liver Diseases
 Drug-Induced Liver Injury Antibodies  >13 publications with DLST and Drug-Induced Liver Injury
 Chest Pain Antibodies  >5 publications with DLST and Chest Pain
 Hepatitis Antibodies  >5 publications with DLST and Hepatitis
 Heart Diseases Antibodies  >4 publications with DLST and Heart Diseases
 Movement Disorders Antibodies  >4 publications with DLST and Movement Disorders
 Exanthema Antibodies  >4 publications with DLST and Exanthema
 Vasculitis Antibodies  >4 publications with DLST and Vasculitis
 Neurobehavioral Manifestations Antibodies  >4 publications with DLST and Neurobehavioral Manifestations

Organs/Tissues associated with DLST recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >37 publications with DLST and Lung
 Brain Antibodies  >31 publications with DLST and Brain
 Blood Antibodies  >29 publications with DLST and Blood
 Liver Antibodies  >20 publications with DLST and Liver
 Skin Antibodies  >12 publications with DLST and Skin
 Muscle Antibodies  >6 publications with DLST and Muscle
 Stomach Antibodies  >5 publications with DLST and Stomach
 Ganglia Antibodies  >3 publications with DLST and Ganglia
 Kidney Antibodies  >2 publications with DLST and Kidney
 Heart Antibodies  >2 publications with DLST and Heart
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