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Nucleobindin-2 (NUCB2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144766

Description: The Recombinant Human NUCB2 (Nesfatin) produced in E Coli has a molecular mass of 10.79kDa containing 92 amino acid residues of the human NUCB2 and fused to a 10 a.a. His tag at N-terminus.Introduction: Nucleobindin-2 (also known as NUCB2 or… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleobindin-2 (NUCB2), Recombinant Protein
Full Product Name
Recombinant Human Nucleobindin-2, His Tag
Product Gene Name
Product Synonym Names
NUCB2 Human, His; Nucleobindin-2 Human Recombinant, His Tag; Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NUCB2; NEFA; Nesfatin; NUCB2, His
Sequence
MKHHHHHHAS VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
Host
E Coli
Form/Format
NUCB2 (Nesfatin) was filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM Tris and 50mM NaCl, pH 7.5.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Molecular Weight
50,201 Da
OMIM
608020
3D Structure
ModBase 3D Structure for P80303
Solubility
It is recomended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications Tested/Suitable for NUCB2 recombinant protein
Western Blot (WB)

Other Notes

Small volumes of NUCB2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUCB2 purchase

MBS144766 is a recombinant protein targeting NUCB2. Also known as Recombinant Human Nucleobindin-2, His Tag. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NUCB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
UniProt Protein Name
Nucleobindin-2
UniProt Synonym Protein Names
DNA-binding protein NEFA; Gastric cancer antigen Zg4; PrepronesfatinNesfatin-1
Protein Family
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN
NCBI Protein Information
nucleobindin-2; DNA-binding protein NEFA; epididymis secretory protein Li 109; gastric cancer antigen Zg4; nesfatin 1; nesfatin-1; nucleobinding 2; prepronefastin; prepronesfatin

NCBI Summary for NUCB2

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

UniProt Comments for NUCB2

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; plasma membrane; ER-Golgi intermediate compartment; nuclear envelope; cytosol

Molecular Function: protein binding; DNA binding; calcium ion binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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