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Mediator of RNA Polymerase II Transcription Subunit 12 (MED12) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1448623

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8… More details

$2265 / 0.05 mg (Baculovirus) +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Mediator of RNA polymerase II transcription subunit 12 (MED12), Recombinant Protein
Full Product Name
Recombinant Human Mediator of RNA polymerase II transcription subunit 12 (MED12) , partial
Product Gene Name
MED12 recombinant protein
Product Synonym Gene Name
MED12
Sequence
LAKKLQKELG ERQSDSLEKV RQLLPLPKQT RDVITCEPQG SLIDTKGNKI AGFDSIFKKE GLQVSTKQKI SPWDLFEGLK PSAPLSWGWF GTVRVDRRVA RGEEQQRLLL YHTHLRPRPR AYYLEPLPLP PEDEEPPAPT LLEPEKKAPE PPKTDKPGAA PPSTEERKKK STKGKKRSQP ATKTEDYGMG PGRSGPYGVT VPPDLLHHPN PGSITHLNYR QGSIGLYTQN QPLPAGGPRV DPYRPVRLPM QKLPTRPTYP GVLPTTMTGV MGLEPSSYKT SVYRQQQPAV PQGQRLRQQL QQSQGMLGQS SVHQMTPSSS YGLQTSQGYT PYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MISTMTPMSA QGVQAGVRST AILPEQ
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
242,953 Da
Sequence Positions
1616-2051. Partial, provide the Interaction with CTNNB1 and GLI3 Region
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
300188
3D Structure
ModBase 3D Structure for Q93074
Species
Human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MED12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED12 purchase

MBS1448623 is a recombinant protein targeting MED12. Also known as Recombinant Human Mediator of RNA polymerase II transcription subunit 12 (MED12) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for MED12. It may not necessarily be applicable to this product.

NCBI GI #
110347429
NCBI GeneID
9968
NCBI Accession #
NP_005111.2
UniProt Primary Accession #
Q93074
UniProt Secondary Accession #
O15410; O75557; Q9UHV6; Q9UND7
UniProt Related Accession #
Q93074
GenBank Nucleotide #
NM_005120.2
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 12
NCBI Official Synonym Full Names
mediator complex subunit 12
NCBI Official Symbol
MED12
NCBI Official Synonym Symbols
OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 12
UniProt Synonym Protein Names
Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED12
UniProt Synonym Gene Names
ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 12

NCBI Summary for MED12

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]
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UniProt Comments for MED12

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
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Research Articles on MED12

1. Data indicate that mediator complex subunit 12 (MED12) plays a central oncogenic role in breast fibroepithelial tumorigenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MED12 recombinant protein

 Reference Product  PubMed Publications
 MED13 recombinant protein  >16 publications with MED12 and MED13
 CDK8 recombinant protein  >14 publications with MED12 and CDK8
 SOX9 recombinant protein  >4 publications with MED12 and SOX9
 MED1 recombinant protein  >1 publications with MED12 and MED1

Pathways associated with MED12 recombinant protein

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with MED12 recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with MED12 and Nervous System Diseases
 Neurobehavioral Manifestations Antibodies  >14 publications with MED12 and Neurobehavioral Manifestations
 Opitz-Kaveggia syndrome Antibodies  >7 publications with MED12 and Opitz-Kaveggia syndrome
 Muscle Hypotonia Antibodies  >7 publications with MED12 and Muscle Hypotonia
 Macrocephaly Antibodies  >6 publications with MED12 and Macrocephaly
 Brain Diseases Antibodies  >4 publications with MED12 and Brain Diseases
 Necrosis Antibodies  >2 publications with MED12 and Necrosis
 Prostatic Diseases Antibodies  >2 publications with MED12 and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >2 publications with MED12 and Prostatic Neoplasms
 Fetal Death Antibodies  >1 publications with MED12 and Fetal Death

Organs/Tissues associated with MED12 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >27 publications with MED12 and Thyroid
 Muscle Antibodies  >18 publications with MED12 and Muscle
 Brain Antibodies  >10 publications with MED12 and Brain
 Blood Antibodies  >8 publications with MED12 and Blood
 Eye Antibodies  >5 publications with MED12 and Eye
 Prostate Antibodies  >4 publications with MED12 and Prostate
 Uterus Antibodies  >3 publications with MED12 and Uterus
 Embryonic Tissue Antibodies  >2 publications with MED12 and Embryonic Tissue
 Connective Tissue Antibodies  >1 publications with MED12 and Connective Tissue
 Bladder Antibodies  >1 publications with MED12 and Bladder
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