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Phenylalanyl-Trna Synthetase 2 (FARS2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144878

Description: FARS2 produced in E Coli is a single, non-glycosylated polypeptide chain containing 436 amino acids (37-451 a.a.) and having a molecular mass of 50.6kDa.FARS2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Phenylalanyl-tRNA Synthetase 2 (FARS2), Recombinant Protein
Full Product Name
Recombinant Human Phenylalanyl-tRNA Synthetase 2
Product Gene Name
Product Synonym Names
FARS2 Human; Phenylalanyl-tRNA Synthetase 2 Human Recombinant; Phenylalanyl-tRNA synthetase mitochondrial; Phenylalanine--tRNA ligase; PheRS; FARS2; FARS1; HSPC320; dJ520B18.2
Sequence
MGSSHHH HHH SSGLVPRGSH MPAAEC ATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHP LWLIKERVKE HFYKQYVGRF GTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEW LEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF
Host
E Coli
Form/Format
FARS2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol, 1mM EDTA and 0.1M NaCl.
Sterile filtered colorless solution.
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Molecular Weight
52,357 Da
OMIM
611592
3D Structure
ModBase 3D Structure for O95363

Product Categories/Family for FARS2 recombinant protein

Other Notes

Small volumes of FARS2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FARS2 purchase

MBS144878 is a recombinant protein targeting FARS2. Also known as Recombinant Human Phenylalanyl-tRNA Synthetase 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for FARS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phenylalanine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
phenylalanyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
FARS2
NCBI Official Synonym Symbols
FARS1; PheRS; COXPD14; HSPC320; dJ520B18.2
UniProt Protein Name
Phenylalanine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Phenylalanyl-tRNA synthetase; PheRS
UniProt Gene Name
FARS2
UniProt Synonym Gene Names
FARS1; PheRS
UniProt Entry Name
SYFM_HUMAN
NCBI Protein Information
phenylalanine--tRNA ligase, mitochondrial; dJ236A3.1 (phenylalanine-tRNA synthetase); dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase)); phenylalanine tRNA ligase 2, mitochondrial; phenylalanine translase; phenylalanine-tRNA synthetase 1 (mitochondrial)

NCBI Summary for FARS2

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. [provided by RefSeq, Jul 2008]
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UniProt Comments for FARS2

FARS2: Catalyzes direct attachment of p-Tyr (Tyr) to tRNAPhe. Permits also, with a lower efficiency, the attachment of m-Tyr to tRNAPhe, thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.20; RNA-binding; Translation; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: mitochondrial matrix

Molecular Function: phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; tRNA processing; phenylalanyl-tRNA aminoacylation; gene expression

Disease: Combined Oxidative Phosphorylation Deficiency 14
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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