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ALKB Alkylation Repair Homolog 2 (ALKBH2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144905

Description: ALKBH2 produced in E Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31.4kDa.ALKBH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ALKB Alkylation Repair Homolog 2 (ALKBH2), Recombinant Protein
Full Product Name
Recombinant Human ALKB Alkylation Repair Homolog 2
Product Synonym Names
ALKBH2 Human; ALKB Alkylation Repair Homolog 2 Human Recombinant; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; Alkylated DNA repair protein alkB homolog 2; Oxy DC1; ALKBH2; ABH2; FLJ99103; MGC90512
Sequence
MGSSHHH HHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K
Host
E Coli
Form/Format
ALKBH2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 30% glycerol.
Sterile filtered colorless solution.
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Molecular Weight
17,083 Da
OMIM
610602
3D Structure
ModBase 3D Structure for Q6NS38

Product Categories/Family for ALKBH2 recombinant protein

Other Notes

Small volumes of ALKBH2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALKBH2 purchase

MBS144905 is a recombinant protein targeting ALKBH2. Also known as Recombinant Human ALKB Alkylation Repair Homolog 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 isoform 1
NCBI Official Synonym Full Names
alkB, alkylation repair homolog 2 (E. coli)
NCBI Official Symbol
ALKBH2
NCBI Official Synonym Symbols
ABH2
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
UniProt Synonym Protein Names
Alkylated DNA repair protein alkB homolog 2; DNA oxidative demethylase ALKBH2; Oxy DC1
UniProt Gene Name
ALKBH2
UniProt Synonym Gene Names
ABH2
UniProt Entry Name
ALKB2_HUMAN
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; 2OG-Fe(II) oxy DC1; DNA oxidative demethylase ALKBH2; alkylated DNA repair protein alkB homolog 2

NCBI Summary for ALKBH2

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ALKBH2

ALKBH2: Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.33; Demethylase; DNA repair, damage; Hydrolase; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function: ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity

Biological Process: DNA dealkylation; DNA repair
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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