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Endonuclease-III (NTH) E Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144942

Description: NTH E Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endonuclease-III (NTH), Recombinant Protein
Full Product Name
Recombinant E Coli Endonuclease-III
Product Gene Name
Product Synonym Names
NTH E.Coli; Endonuclease-III E.Coli Recombinant; DNA-(apurinic or apyrimidinic site) lyase; b1633; JW1625
Sequence
MGSSHHH HHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE   EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
Host
E Coli
Form/Format
The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 85.0% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
23,562 Da
3D Structure
ModBase 3D Structure for P0AB83

Product Categories/Family for NTH recombinant protein

Other Notes

Small volumes of NTH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NTH purchase

MBS144942 is a recombinant protein targeting NTH. Also known as Recombinant E Coli Endonuclease-III. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NTH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
NCBI Official Symbol
nth
NCBI Official Synonym Symbols
ECK1629; JW1625
UniProt Protein Name
Endonuclease III
UniProt Synonym Protein Names
DNA-(apurinic or apyrimidinic site) lyase
Protein Family
UniProt Gene Name
nth
UniProt Entry Name
END3_ECOLI
NCBI Protein Information
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)

NCBI Summary for NTH

Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].
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UniProt Comments for NTH

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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