Rabbit raTNF a Active Protein – Cat# MBS145048

Tumor Necrosis Factor-Alpha (raTNF a) Rabbit Active Protein

Name: raTNF a Active Protein; Tumor Necrosis Factor-Alpha; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS145048
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145048

Description: Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E Coli is a non-glycosylated, polypeptide chain having a… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tumor Necrosis Factor-alpha (raTNF a), Active Protein

Full Product Name

Recombinant Rabbit Tumor Necrosis Factor-alpha

Product Gene Name

raTNF a active protein

Product Synonym Names

TNF a Rabbit; Tumor Necrosis Factor-Alpha Rabbit Recombinant; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF; TNFA; TNFSF2

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Al a-Ser-Arg

Host

E Coli

Form/Format

TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TNF-a should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

25,817 Da

3D Structure

ModBase 3D Structure for P04924

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.

Product Categories/Family for raTNF a active protein

Cytokines and Growth Factors; Cytokines; Tumor Necrosis Factor

Other Notes

Small volumes of raTNF a active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for raTNF a purchase

MBS145048 is an active protein targeting raTNF a. Also known as Recombinant Rabbit Tumor Necrosis Factor-alpha. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for raTNF a. It may not necessarily be applicable to this product.

NCBI GI #

126722916

NCBI GeneID

100009088

NCBI Accession #

NP_001075732.1

UniProt Primary Accession #

P04924

UniProt Related Accession #

P04924

GenBank Nucleotide #

NM_001082263.1

NCBI Official Full Name

tumor necrosis factor

NCBI Official Symbol

TNF

NCBI Official Synonym Symbols

TNFA; TNFSF2

UniProt Protein Name

Tumor necrosis factor

UniProt Synonym Protein Names

Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Protein Family

Tumor necrosis factor alpha-induced protein

UniProt Gene Name

TNF

UniProt Synonym Gene Names

TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

UniProt Entry Name

TNFA_RABIT

NCBI Protein Information

tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2

UniProt Comments for raTNF a

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Research Articles on raTNF a

1. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with raTNF a active protein

Reference Product	PubMed Publications
VCAM-1 active protein	>2551 publications with raTNF a and VCAM-1
PTGS2 active protein	>2256 publications with raTNF a and PTGS2
CD40 active protein	>1975 publications with raTNF a and CD40
TGF-beta-1 active protein	>1832 publications with raTNF a and TGF-beta-1
IL6 active protein	>1473 publications with raTNF a and IL6
IL8 active protein	>434 publications with raTNF a and IL8
eNOS active protein	>379 publications with raTNF a and eNOS
REL active protein	>312 publications with raTNF a and REL

Pathways associated with raTNF a active protein

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Diseases associated with raTNF a active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>90780 publications with raTNF a and Necrosis
Inflammation Antibodies	>32806 publications with raTNF a and Inflammation
Vascular Diseases Antibodies	>7585 publications with raTNF a and Vascular Diseases
Lung Diseases Antibodies	>6757 publications with raTNF a and Lung Diseases
Liver Diseases Antibodies	>5161 publications with raTNF a and Liver Diseases
Insulin Resistance Antibodies	>2522 publications with raTNF a and Insulin Resistance
Fibrosis Antibodies	>2492 publications with raTNF a and Fibrosis
Atherosclerosis Antibodies	>2416 publications with raTNF a and Atherosclerosis
Colitis Antibodies	>2413 publications with raTNF a and Colitis
Pain Antibodies	>2278 publications with raTNF a and Pain