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Monocyte Chemotactic Protein-5 (MCP 5) Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145071

Description: MCP-5 Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 82 amino acids and having a molecular mass of 9.3kDa. The MCP5 is purified by proprietary chromatographic techniques.Introduction: MCP-5… More details

$250 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Monocyte Chemotactic Protein-5 (MCP 5), Active Protein
Full Product Name
Recombinant Mouse Monocyte Chemotactic Protein-5 (CCL12)
Product Gene Name
MCP 5 active protein
Product Synonym Names
MCP 5 Mouse; Monocyte Chemotactic Protein-5 Mouse Recombinant (CCL12); C-C motif chemokine 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12; Ccl12; Mcp5; Scya12
Sequence
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in 1xPBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized CCL12 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MCP5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Molecular Weight
11,659 Da
3D Structure
ModBase 3D Structure for Q62401
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized MCP5 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.

Product Categories/Family for MCP 5 active protein

Chemokines; MCP

Other Notes

Small volumes of MCP 5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCP 5 purchase

MBS145071 is an active protein targeting MCP 5. Also known as Recombinant Mouse Monocyte Chemotactic Protein-5 (CCL12). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MCP 5. It may not necessarily be applicable to this product.

NCBI GI #
119226206
NCBI GeneID
20293
NCBI Accession #
NP_035461.2
UniProt Primary Accession #
Q62401
UniProt Secondary Accession #
Q9QYD6
UniProt Related Accession #
Q62401
GenBank Nucleotide #
NM_011331.2
NCBI Official Full Name
C-C motif chemokine 12
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 12
NCBI Official Symbol
Ccl12
NCBI Official Synonym Symbols
MCP-5; Scya12
UniProt Protein Name
C-C motif chemokine 12
UniProt Synonym Protein Names
MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12
Protein Family
C-C motif chemokine
UniProt Gene Name
Ccl12
UniProt Synonym Gene Names
Mcp5; Scya12; MCP-5
UniProt Entry Name
CCL12_MOUSE
NCBI Protein Information
C-C motif chemokine 12; MCP-1-related chemokine; monocyte chemoattractant protein 5; monocyte chemotactic protein 5; small inducible cytokine A12; small-inducible cytokine A12

UniProt Comments for MCP 5

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: chemokine activity; CCR2 chemokine receptor binding; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte migration; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration
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Research Articles on MCP 5

1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MCP 5 active protein

 Products by Pathway  Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram

Diseases associated with MCP 5 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >36 publications with MCP 5 and Inflammation
 Disease Models, Animal Antibodies  >22 publications with MCP 5 and Disease Models, Animal
 Respiratory Tract Diseases Antibodies  >16 publications with MCP 5 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >15 publications with MCP 5 and Lung Diseases
 Fibrosis Antibodies  >13 publications with MCP 5 and Fibrosis
 Necrosis Antibodies  >10 publications with MCP 5 and Necrosis
 Hypersensitivity Antibodies  >9 publications with MCP 5 and Hypersensitivity
 Pulmonary Fibrosis Antibodies  >9 publications with MCP 5 and Pulmonary Fibrosis
 Autoimmune Diseases Antibodies  >8 publications with MCP 5 and Autoimmune Diseases
 Neoplasms, Experimental Antibodies  >5 publications with MCP 5 and Neoplasms, Experimental

Organs/Tissues associated with MCP 5 active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with MCP 5 and Brain
 Spleen Antibodies  >5 publications with MCP 5 and Spleen
 Thymus Antibodies  >4 publications with MCP 5 and Thymus
 Embryonic Tissue Antibodies  >2 publications with MCP 5 and Embryonic Tissue
 Prostate Antibodies  >2 publications with MCP 5 and Prostate
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