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Interleukin-22 (mIL 22A) Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145075

Description: Interleukin-22 Antagonist Mouse Recombinant produced in E Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and having a total molecular mass of 16.7 KDa. The Mouse IL-22 Antagonist is purified by… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Interleukin-22 (mIL 22A), Active Protein
Full Product Name
Recombinant Mouse Interleukin-22 Antagonist (E117A)
Product Gene Name
Product Synonym Names
IL-22 Antagonist Mouse; Interleukin-22 Antagonist (E117A) Mouse Recombinant; IL-TIF; TIFa; IL-10-related T-cell-derived-inducible factor; IL-22; ILTIF; IL-D110; zcyto18; MGC79382; MGC79384; TIFIL-23
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pr o-Val-Asn
Host
E Coli
Form/Format
IL-22 Antagonist Mouse is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by: (a) Gel filtration chromatography under non-denaturing conditions. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Preparation and Storage
Lyophilized Interleukin-22 Mouse Antagonist although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL22 Antagonist should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Molecular Weight
20,106 Da
3D Structure
ModBase 3D Structure for Q9JJY9
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized Interleukin -22 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
IL-22 E117A mutant is capable of full inhibition of STAT3 phosphorylation induced by mouse interleukin 22 in HepG cells. Its affinity toward immobilized mIL-22 receptor a1 extracellular domain (mIL-22 Ra1-ECD) or IL-22 binding protein is similar to the non-mutated mouse interleukin 22. Mouse IL-22 antagonist (E117A) has very low agonistic activity in this bioassay.

Other Notes

Small volumes of mIL 22A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mIL 22A purchase

MBS145075 is an active protein targeting mIL 22A. Also known as Recombinant Mouse Interleukin-22 Antagonist (E117A). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mIL 22A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-22
NCBI Official Synonym Full Names
interleukin 22
NCBI Official Symbol
Il22
NCBI Official Synonym Symbols
IL-22; Iltif; IL-22a; ILTIFa
UniProt Protein Name
Interleukin-22
UniProt Synonym Protein Names
IL-10-related T-cell-derived-inducible factor; IL-TIF; IL-TIF alpha; Interleukin-22a; IL-22a
UniProt Gene Name
Il22
UniProt Synonym Gene Names
Il22a; Iltif; Iltifa; IL-22; IL-TIF; IL-22a
UniProt Entry Name
IL22_MOUSE
NCBI Protein Information
interleukin-22; IL-10-related T-cell-derived-inducible factor; IL-TIF alpha; ILTIF alpha; interleukin 10-related T cell-derived inducible factor; interleukin-22a

UniProt Comments for mIL 22A

IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; cytokine activity

Biological Process: negative regulation of inflammatory response; regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with mIL 22A active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with mIL 22A and Brain
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