Human NTHL1 Recombinant Protein – Cat# MBS145085

Nth Endonuclease III-Like 1 (NTHL1) Human Recombinant Protein

Name: NTHL1 Recombinant Protein; Nth Endonuclease III-Like 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS145085
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145085

Description: NTHL1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 332 amino acids (1-312 a.a.) and having a molecular mass of 36.6kDa.NTHL1 is fused to a 20 amino acid His-tag at N-terminus & purified by… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

nth Endonuclease III-Like 1 (NTHL1), Recombinant Protein

Full Product Name

Recombinant Human nth Endonuclease III-Like 1

Product Gene Name

NTHL1 recombinant protein

Product Synonym Names

NTHL1 Human; Nth Endonuclease III-Like 1 Human Recombinant; Endonuclease III-like protein 1; NTHL1; NTH1; OCTS3

Sequence

MGSSHHH HHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL

Host

E Coli

Form/Format

NTHL1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Sterile filtered colorless solution.

Purity/Purification

Greater than 80.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Molecular Weight

32,839 Da

OMIM

602656

3D Structure

ModBase 3D Structure for P78549

Product Categories/Family for NTHL1 recombinant protein

Enzymes; Endonuclease

Other Notes

Small volumes of NTHL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NTHL1 purchase

MBS145085 is a recombinant protein targeting NTHL1. Also known as Recombinant Human nth Endonuclease III-Like 1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NTHL1. It may not necessarily be applicable to this product.

NCBI GI #

4505471

NCBI GeneID

4913

NCBI Accession #

NP_002519.1

UniProt Primary Accession #

P78549

UniProt Secondary Accession #

Q1MVR1; Q99566; Q99794; Q9BPX2

UniProt Related Accession #

P78549

GenBank Nucleotide #

NM_002528.5

NCBI Official Full Name

endonuclease III-like protein 1

NCBI Official Synonym Full Names

nth endonuclease III-like 1 (E. coli)

NCBI Official Symbol

NTHL1

NCBI Official Synonym Symbols

NTH1; OCTS3; hNTH1

UniProt Protein Name

Endonuclease III-like protein 1

UniProt Synonym Protein Names

Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase

Protein Family

Endonuclease III-like protein

UniProt Gene Name

NTHL1

UniProt Synonym Gene Names

hNTH1

UniProt Entry Name

NTH_HUMAN

NCBI Protein Information

endonuclease III-like protein 1; DNA glycoslyase/AP lyase; NTHL1; bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase

NCBI Summary for NTHL1

The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008]

UniProt Comments for NTHL1

NTHL1: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Belongs to the Nth/MutY family.

Protein type: DNA repair, damage; Hydrolase; Mitochondrial; EC 4.2.99.18; Lyase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; endonuclease activity; metal ion binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; DNA N-glycosylase activity

Biological Process: base-excision repair, AP site formation; base-excision repair; depyrimidination; DNA repair; nucleotide-excision repair, DNA incision, 5'-to lesion; DNA catabolic process, endonucleolytic

Disease: Familial Adenomatous Polyposis 3

Research Articles on NTHL1

1. we demonstrated that hNEIL1 and hNTH1 cleave Oz sites as efficiently as 5-hydroxyuracil sites. Thus, hNEIL1 and hNTH1 can repair Oz lesions

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NTHL1 recombinant protein

Reference Product	PubMed Publications
NEIL1 recombinant protein	>18 publications with NTHL1 and NEIL1
OGG1 recombinant protein	>16 publications with NTHL1 and OGG1
NEIL2 recombinant protein	>9 publications with NTHL1 and NEIL2
APEX1 recombinant protein	>6 publications with NTHL1 and APEX1
NUDT1 recombinant protein	>3 publications with NTHL1 and NUDT1
YBX1 recombinant protein	>2 publications with NTHL1 and YBX1
XPA recombinant protein	>1 publications with NTHL1 and XPA

Pathways associated with NTHL1 recombinant protein

Products by Pathway	Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base-Excision Repair, AP Site Formation Pathway antibodies	Base-Excision Repair, AP Site Formation Pathway Diagram
Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies	Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
Cleavage Of The Damaged Pyrimidine Pathway antibodies	Cleavage Of The Damaged Pyrimidine Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
Depyrimidination Pathway antibodies	Depyrimidination Pathway Diagram
Displacement Of DNA Glycosylase By APE1 Pathway antibodies	Displacement Of DNA Glycosylase By APE1 Pathway Diagram
Recognition And Association Of DNA Glycosylase With Site Containing An Affected Pyrimidine Pathway antibodies	Recognition And Association Of DNA Glycosylase With Site Containing An Affected Pyrimidine Pathway Diagram

Diseases associated with NTHL1 recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>6 publications with NTHL1 and Nervous System Diseases
Congenital Abnormalities Antibodies	>4 publications with NTHL1 and Congenital Abnormalities
Neoplasms, Experimental Antibodies	>2 publications with NTHL1 and Neoplasms, Experimental
Brain Diseases Antibodies	>2 publications with NTHL1 and Brain Diseases
Ovarian Neoplasms Antibodies	>2 publications with NTHL1 and Ovarian Neoplasms
Death Antibodies	>2 publications with NTHL1 and Death
Kidney Diseases Antibodies	>2 publications with NTHL1 and Kidney Diseases
Lung Diseases Antibodies	>2 publications with NTHL1 and Lung Diseases
Liver Neoplasms Antibodies	>1 publications with NTHL1 and Liver Neoplasms
Disease Models, Animal Antibodies	>1 publications with NTHL1 and Disease Models, Animal

Organs/Tissues associated with NTHL1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>4 publications with NTHL1 and Blood
Kidney Antibodies	>3 publications with NTHL1 and Kidney
Liver Antibodies	>3 publications with NTHL1 and Liver
Lung Antibodies	>3 publications with NTHL1 and Lung
Lymph Antibodies	>1 publications with NTHL1 and Lymph
Pharynx Antibodies	>1 publications with NTHL1 and Pharynx
Muscle Antibodies	>1 publications with NTHL1 and Muscle
Testis Antibodies	>1 publications with NTHL1 and Testis
Stomach Antibodies	>1 publications with NTHL1 and Stomach
Brain Antibodies	>1 publications with NTHL1 and Brain