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Polymerase, Lambda (POLL) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145251

Description: POLL Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Polymerase, Lambda (POLL), Recombinant Protein
Full Product Name
Recombinant Human Polymerase (DNA directed), Lambda
Product Gene Name
Product Synonym Names
POLL Human; Polymerase (DNA directed), Lambda Human Recombinant; Polymerase (DNA directed) lambda; Pol beta2; DNA polymerase beta-2; DNA polymerase kappa; BETAN; POLKAPPA; DNA polymerase beta-N; EC 2.7.7.7; EC 4.2.99
Sequence
MGSSHHH HHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW .
Host
E Coli
Form/Format
The POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Molecular Weight
33,839 Da
OMIM
606343
3D Structure
ModBase 3D Structure for Q9UGP5

Product Categories/Family for POLL recombinant protein

Other Notes

Small volumes of POLL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLL purchase

MBS145251 is a recombinant protein targeting POLL. Also known as Recombinant Human Polymerase (DNA directed), Lambda. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase lambda isoform a
NCBI Official Synonym Full Names
polymerase (DNA directed), lambda
NCBI Official Symbol
POLL
NCBI Official Synonym Symbols
BETAN; POLKAPPA
UniProt Protein Name
DNA polymerase lambda
UniProt Synonym Protein Names
DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Protein Family
UniProt Gene Name
POLL
UniProt Synonym Gene Names
Pol Lambda; Pol beta2
UniProt Entry Name
DPOLL_HUMAN
NCBI Protein Information
DNA polymerase lambda; DNA polymerase beta-2; DNA polymerase beta-N; DNA polymerase kappa

NCBI Summary for POLL

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
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UniProt Comments for POLL

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: DNA repair, damage; Transferase; EC 2.7.7.7; Lyase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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