Human NUDT21 Recombinant Protein – Cat# MBS145268

Nudix Type Motif 21 (NUDT21) Human Recombinant Protein

Name: NUDT21 Recombinant Protein; Nudix Type Motif 21; Human; MyBioSource
Brand: MyBioSource
SKU: MBS145268
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145268

Description: NUDT21 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 247 amino acids (1-227 a.a.) and having a molecular mass of 28.3kDa. The NUDT21 is purified… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Nudix Type Motif 21 (NUDT21), Recombinant Protein

Full Product Name

Recombinant Human Nudix Type Motif 21

Product Gene Name

NUDT21 recombinant protein

Product Synonym Names

NUDT21 Human; Nudix Type Motif 21 Human Recombinant; Cleavage and polyadenylation specificity factor subunit 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; CFIm25; CPSF 25 kDa subunit; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Pre-mRNA cleavage factor Im 25 kDa subunit; NUDT21; CFIM25; CPSF25; CPSF5; DKFZp686H1588

Sequence

MGSSHHH HHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN

Host

E Coli

Form/Format

The NUDT21 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 20% glycerol.
Sterile Filtered colorless solution.

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Molecular Weight

26,227 Da

OMIM

604978

3D Structure

ModBase 3D Structure for O43809

Product Categories/Family for NUDT21 recombinant protein

Enzymes; Nudix Type Motif

Other Notes

Small volumes of NUDT21 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUDT21 purchase

MBS145268 is a recombinant protein targeting NUDT21. Also known as Recombinant Human Nudix Type Motif 21. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NUDT21. It may not necessarily be applicable to this product.

NCBI GI #

5901926

NCBI GeneID

11051

NCBI Accession #

NP_008937.1

UniProt Primary Accession #

O43809

UniProt Secondary Accession #

Q6IB85; Q6NE84

UniProt Related Accession #

O43809

GenBank Nucleotide #

NM_007006.2

NCBI Official Full Name

cleavage and polyadenylation specificity factor subunit 5

NCBI Official Synonym Full Names

nudix (nucleoside diphosphate linked moiety X)-type motif 21

NCBI Official Symbol

NUDT21

NCBI Official Synonym Symbols

CPSF5; CFIM25

UniProt Protein Name

Cleavage and polyadenylation specificity factor subunit 5

UniProt Synonym Protein Names

Cleavage and polyadenylation specificity factor 25 kDa subunit; CFIm25; CPSF 25 kDa subunit; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Pre-mRNA cleavage factor Im 25 kDa subunit

Protein Family

Nudix hydrolase

UniProt Gene Name

NUDT21

UniProt Synonym Gene Names

CFIM25; CPSF25; CPSF5; CFIm25; CPSF 25 kDa subunit; Nudix motif 21

UniProt Entry Name

CPSF5_HUMAN

NCBI Protein Information

cleavage and polyadenylation specificity factor subunit 5; CPSF 25 kDa subunit; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specific factor 5, 25 kDa; cleavage and polyadenylation specificity factor 25 kDa subunit; cleavage factor Im complex 25 kDa subunit; nucleoside diphosphate-linked moiety X motif 21; nudix motif 21; pre-mRNA cleavage factor Im (25kD); pre-mRNA cleavage factor Im 25 kDa subunit; pre-mRNA cleavage factor Im, 25kD subunit

NCBI Summary for NUDT21

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008]

UniProt Comments for NUDT21

CFIM25: Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export. Belongs to the Nudix hydrolase family. CPSF5 subfamily.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nucleoplasm; centrosome; paraspeckles; nucleus; mRNA cleavage factor complex

Molecular Function: mRNA binding; protein binding; protein homodimerization activity; hydrolase activity; RNA binding; histone deacetylase binding; AU-rich element binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; RNA splicing; mRNA polyadenylation; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; protein tetramerization

Research Articles on NUDT21

1. Structure of a CFI(m)25/CFI(m)68 RRM heterotetramer and biochemical data indicated that CFIm25 specifically recognized two UGUA elements, CFIm68 facilitates looping of the intervening RNA. CFIm-mediated RNA looping may regulate alternative polyadnylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NUDT21 recombinant protein

Reference Product	PubMed Publications
CPSF6 recombinant protein	>2 publications with NUDT21 and CPSF6

Pathways associated with NUDT21 recombinant protein

Products by Pathway	Pathway Diagram
Cleavage Of Growing Transcript In The Termination Region Pathway antibodies	Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Processing Of Capped Intronless Pre-mRNA Pathway antibodies	Processing Of Capped Intronless Pre-mRNA Pathway Diagram
Processing Of Intronless Pre-mRNAs Pathway antibodies	Processing Of Intronless Pre-mRNAs Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
RNA Polymerase II Transcription Termination Pathway antibodies	RNA Polymerase II Transcription Termination Pathway Diagram
MRNA 3'-end Processing Pathway antibodies	MRNA 3'-end Processing Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram

Diseases associated with NUDT21 recombinant protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>1 publications with NUDT21 and Immune System Diseases

Organs/Tissues associated with NUDT21 recombinant protein

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with NUDT21 and Testis