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Nucleoside Diphosphate Kinase (NDK) E Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145412

NDK E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-143 a.a.) and having a molecular mass of 18kDa.NDK is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleoside Diphosphate Kinase (NDK), Recombinant Protein
Full Product Name
Recombinant E Coli Nucleoside Diphosphate Kinase
Product Gene Name
Product Synonym Names
NDK E.Coli; Nucleoside Diphosphate Kinase E.Coli Recombinant; Nucleoside diphosphate kinase; NDK; NDP kinase; Nucleoside-2-P kinase; b2518; JW2502
Sequence
MGSSHHH HHH SSGLVPRGSH MGSHMA IERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR.
Host
E Coli
Form/Format
NDK protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
3D Structure
ModBase 3D Structure for P0A763
Physical Appearance
Sterile Filtered colorless solution.

Other Notes

Small volumes of NDK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDK purchase

MBS145412 is a recombinant protein targeting NDK. Also known as Recombinant E Coli Nucleoside Diphosphate Kinase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NDK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase
NCBI Official Symbol
ndk
NCBI Official Synonym Symbols
ECK2514; JW2502
UniProt Protein Name
Nucleoside diphosphate kinase
UniProt Synonym Protein Names
Nucleoside-2-P kinase
UniProt Gene Name
ndk
UniProt Synonym Gene Names
NDK; NDP kinase
UniProt Entry Name
NDK_ECOLI
NCBI Protein Information
multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase

NCBI Summary for NDK

Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG10650]. Nucleoside diphosphate kinase catalyzes the reaction in which the terminal phosphate of a nucleoside-triphosphate is transferred to a nucleoside-diphosphate. [More information is available at EcoCyc: EG10650].

UniProt Comments for NDK

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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