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DNA Topoisomerase-I (bTOP1) Bovine Native Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145448

Description: Bovine DNA Topoisomerase-I purified from bovine tissues by proprietary chromatographic techniques.Introduction: TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DNA Topoisomerase-I (bTOP1), Native Protein
Full Product Name
Bovine DNA Topoisomerase-I
Product Gene Name
Product Synonym Names
TOP1 Bovine; DNA Topoisomerase-I Bovine; DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70
Host
Bovine tissues
Form/Format
TOP1 is supplied at a concentration of 1mg/1ml in 16mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.
Purity/Purification
Greater than 70% as determined by SDS-PAGE.
Concentration
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. (lot specific)
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight
7,728 Da
3D Structure
ModBase 3D Structure for Q2HPH0
Immunological Functions
1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels) and immunodot test with positive/negative sera panels.

Product Categories/Family for bTOP1 native protein

Applications Tested/Suitable for bTOP1 native protein
Western Blot (WB)
Application Notes for bTOP1 native protein
Western blot with Scleroderma positive patient sera.

Other Notes

Small volumes of bTOP1 native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for bTOP1 purchase

MBS145448 is a native protein targeting bTOP1. Also known as Bovine DNA Topoisomerase-I. Host: Bovine tissues.

Additional Details

NCBI/Uniprot data below describe general gene information for bTOP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Topoisomerase I
UniProt Protein Name
Topoisomerase I
UniProt Synonym Protein Names
Topoisomerase I
Protein Family
UniProt Entry Name
Q2HPH0_BOVIN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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