Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) Human Recombinant Protein

SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.-; DNA repair, damage; Hydrolase

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; protein binding; uracil DNA N-glycosylase activity; DNA binding; oxidized pyrimidine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity

Biological Process: base-excision repair, AP site formation; depyrimidination; base-excision repair; DNA repair; DNA catabolic process, endonucleolytic