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Leukemia Inhibitory Factor (rLIF) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145977

Description: Leukemia Inhibitory Factor (LIF) Rat Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.8 kDa. The Leukemia Inhibitory Factor (LIF) is purified by… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Leukemia Inhibitory Factor (rLIF), Active Protein
Full Product Name
Recombinant Rat Leukemia Inhibitory Factor
Product Gene Name
Product Synonym Names
LIF Rat; Leukemia Inhibitory Factor Rat Recombinant; Leukemia inhibitory factor; Cholinergic neuronal differentiation factor; Lif
Sequence
SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF
Host
E Coli
Form/Format
LIF Rat was lyophilized from 0.2 um filtered concentrated solution in 1xPBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
22,113 Da
3D Structure
ModBase 3D Structure for P17777
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/mL.

Other Notes

Small volumes of rLIF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rLIF purchase

MBS145977 is an active protein targeting rLIF. Also known as Recombinant Rat Leukemia Inhibitory Factor. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for rLIF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Leukemia inhibitory factor
NCBI Official Synonym Full Names
leukemia inhibitory factor
NCBI Official Symbol
Lif
UniProt Protein Name
Leukemia inhibitory factor
UniProt Synonym Protein Names
Cholinergic neuronal differentiation factor
UniProt Gene Name
Lif
UniProt Synonym Gene Names
LIF
UniProt Entry Name
LIF_RAT
NCBI Protein Information
leukemia inhibitory factor; cholinergic neuronal differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor)

NCBI Summary for rLIF

a secreted cytokine; involved in embryonic stem cell and myeloid cell growth and differentiation and stimulation of bone remodeling [RGD, Feb 2006]

UniProt Comments for rLIF

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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