Human GOT2 Recombinant Protein – Cat# MBS146276

Glutamic-Oxaloacetic Transaminase 2 (GOT2) Human Recombinant Protein

Name: GOT2 Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS146276
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146276

Description: GOT2 Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (30-430 a.a.) and having a molecular mass of 47kDa. The GOT2 fused to a 23 amino acid his tag at N-terminus and is purified by… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Glutamic-Oxaloacetic Transaminase 2 (GOT2), Recombinant Protein

Full Product Name

Recombinant Human Glutamic-Oxaloacetic Transaminase 2

Product Gene Name

GOT2 recombinant protein

Product Synonym Names

GOT2 Human; Glutamic-Oxaloacetic Transaminase 2 Human Recombinant; EC 2.6.1.1; Aspartate aminotransferase 1; Transaminase A; GIG18

Sequence

MGSSHHH HHH SSGLVPRGSH MGSSSW WTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFD FTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHN WQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Host

E Coli

Form/Format

The GOT2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Sterile filtered colorless solution.

Purity/Purification

Greater than 95.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Molecular Weight

43,030 Da

OMIM

138150

3D Structure

ModBase 3D Structure for P00505

Product Categories/Family for GOT2 recombinant protein

Enzymes; Transaminase

Other Notes

Small volumes of GOT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT2 purchase

MBS146276 is a recombinant protein targeting GOT2. Also known as Recombinant Human Glutamic-Oxaloacetic Transaminase 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT2. It may not necessarily be applicable to this product.

NCBI GI #

555290167

NCBI GeneID

2806

NCBI Accession #

NP_001273149.1

UniProt Primary Accession #

P00505

UniProt Secondary Accession #

Q53FL3; Q9BWA3; B4DJA6; E7ERW2

UniProt Related Accession #

P00505

GenBank Nucleotide #

NM_001286220.1

NCBI Official Full Name

aspartate aminotransferase, mitochondrial isoform 2

NCBI Official Synonym Full Names

glutamic-oxaloacetic transaminase 2, mitochondrial

NCBI Official Symbol

GOT2

NCBI Official Synonym Symbols

KAT4; KATIV; mitAAT

UniProt Protein Name

Aspartate aminotransferase, mitochondrial

UniProt Synonym Protein Names

Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A

UniProt Gene Name

GOT2

UniProt Synonym Gene Names

mAspAT; FABP-1; FABPpm

UniProt Entry Name

AATM_HUMAN

NCBI Protein Information

aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; aspartate aminotransferase 2; aspartate transaminase 2; fatty acid-binding protein; glutamate oxaloacetate transaminase 2; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); kynurenine aminotransferase 4; kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mAspAT; plasma membrane-associated fatty acid-binding protein; transaminase A

NCBI Summary for GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

UniProt Comments for GOT2

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.1; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.7; Mitochondrial; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; fatty acid transport

Research Articles on GOT2

1. High glucose infusion was associated with increased expression of higher plasma membrane-associated fatty acid-binding protein (FABPpm).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GOT2 recombinant protein

Reference Product	PubMed Publications
GOT1 recombinant protein	>7 publications with GOT2 and GOT1
MDH2 recombinant protein	>4 publications with GOT2 and MDH2
MDH1 recombinant protein	>3 publications with GOT2 and MDH1
IDH2 recombinant protein	>2 publications with GOT2 and IDH2

Pathways associated with GOT2 recombinant protein

Products by Pathway	Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
4-hydroxyproline Degradation Pathway antibodies	4-hydroxyproline Degradation Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram

Diseases associated with GOT2 recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>3 publications with GOT2 and Disease Models, Animal
Mental Disorders Antibodies	>2 publications with GOT2 and Mental Disorders
Cardiovascular Diseases Antibodies	>2 publications with GOT2 and Cardiovascular Diseases
Cardiomyopathies Antibodies	>1 publications with GOT2 and Cardiomyopathies
Liver Diseases Antibodies	>1 publications with GOT2 and Liver Diseases
Fatty Liver Antibodies	>1 publications with GOT2 and Fatty Liver
Hypertension Antibodies	>1 publications with GOT2 and Hypertension
Nervous System Diseases Antibodies	>1 publications with GOT2 and Nervous System Diseases
Heart Diseases Antibodies	>1 publications with GOT2 and Heart Diseases

Organs/Tissues associated with GOT2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>8 publications with GOT2 and Blood
Thyroid Antibodies	>3 publications with GOT2 and Thyroid
Muscle Antibodies	>3 publications with GOT2 and Muscle
Liver Antibodies	>3 publications with GOT2 and Liver
Brain Antibodies	>3 publications with GOT2 and Brain
Vascular Antibodies	>2 publications with GOT2 and Vascular
Heart Antibodies	>2 publications with GOT2 and Heart
Adrenal Gland Antibodies	>1 publications with GOT2 and Adrenal Gland
Intestine Antibodies	>1 publications with GOT2 and Intestine
Kidney Antibodies	>1 publications with GOT2 and Kidney