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Cyclophilin-H (PPIH) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146277

Description: PPIH Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (a.a 2-177) containing 186 amino acids and including a 10 a.a N-terminal His tag. The total molecular mass is 20.3kDa (calculated).Introduction: PPIH is a… More details

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Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cyclophilin-H (PPIH), Recombinant Protein
Full Product Name
Recombinant Human Cyclophilin-H, His Tag
Product Gene Name
PPIH recombinant protein
Product Synonym Names
PPIH Human, His; Cyclophilin-H Human Recombinant, His Tag; Oeptidylprolyl Isomerase H; PPIH; CYPH; CYP20; SnuCyp-20; Peptidyl-prolyl cis-trans isomerase H; PPIase H; Rotamase H; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP; Small nuclear ribonucleoprotein particle-specific cyclophilin H; peptidylprolyl isomerase H; CYP-20; MGC5016; Cyclophilin-H; PPIH His
Sequence
MKHHHHH HASAVA NSSPVNP VVFFDVSIGG QEVGRMKIEL FADVVPKTAE NFRQFCTGEF RKDGVPIGYK GSTFHRVIKD FMIQGGDFVN GDGTGVASIY RGPFADENFK LRHSAPGLLS MANSGPSTNG CQFFITCSKC DWLDGKHVVF GKIIDGLLVM RKIENVPTGP NNKPKLPVVI SQCGEM
Host
E Coli
Form/Format
Filtered (0.4 um) and lyophilized from 0.5 mg/ml in phosphate buffered saline pH 7.4.
Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Purity as determined by densitometric image analysis is greater than 95%.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Molecular Weight
14,492 Da
OMIM
606095
3D Structure
ModBase 3D Structure for O43447
Solubility
It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. PPIH is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Product Categories/Family for PPIH recombinant protein

Enzymes; Cyclophilin

Other Notes

Small volumes of PPIH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIH purchase

MBS146277 is a recombinant protein targeting PPIH. Also known as Recombinant Human Cyclophilin-H, His Tag. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIH. It may not necessarily be applicable to this product.

NCBI GI #
5454154
NCBI GeneID
10465
NCBI Accession #
NP_006338.1
UniProt Primary Accession #
O43447
UniProt Secondary Accession #
A6NNE7
UniProt Related Accession #
O43447
GenBank Nucleotide #
NM_006347.3
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase H
NCBI Official Synonym Full Names
peptidylprolyl isomerase H (cyclophilin H)
NCBI Official Symbol
PPIH
NCBI Official Synonym Symbols
CYPH; CYP-20; USA-CYP; SnuCyp-20
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase H
UniProt Synonym Protein Names
Rotamase H; Small nuclear ribonucleoprotein particle-specific cyclophilin H; CypH; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP
Protein Family
Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name
PPIH
UniProt Synonym Gene Names
CYP20; CYPH; PPIase H; CypH; USA-CYP
UniProt Entry Name
PPIH_HUMAN
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase H; PPIase h; U-snRNP-associated cyclophilin SnuCyp-20; U-snRNP-associated cyclophilin SunCyp-20; USA-CyP SnuCyp-20; cyclophilin H; rotamase H; small nuclear ribonucleoprotein particle-specific cyclophilin H

NCBI Summary for PPIH

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPIH

PPIH: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone. Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.

Protein type: Spliceosome; RNA processing; EC 5.2.1.8; Isomerase; Cyclophilin

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: spliceosome; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck

Molecular Function: ribonucleoprotein binding; protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding

Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; positive regulation of viral genome replication; protein folding; protein complex assembly
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Research Articles on PPIH

1. Free and complexed cyclophilin H have virtually identical conformations suggesting that the U4/U6-60K binding site is pre-shaped and the peptidyl-prolyl-cis/trans isomerase activity is unaffected by complex formation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PPIH recombinant protein

 Products by Pathway  Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies  Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram
 Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies  Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram

Diseases associated with PPIH recombinant protein

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >7 publications with PPIH and Hypertension
 Fetal Death Antibodies  >1 publications with PPIH and Fetal Death
 Cardiovascular Diseases Antibodies  >1 publications with PPIH and Cardiovascular Diseases

Organs/Tissues associated with PPIH recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with PPIH and Blood
 Vascular Antibodies  >6 publications with PPIH and Vascular
 Uterus Antibodies  >5 publications with PPIH and Uterus
 Umbilical Cord Antibodies  >3 publications with PPIH and Umbilical Cord
 Placenta Antibodies  >1 publications with PPIH and Placenta
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