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Staphylococcal Protein-A (SPA) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146582

Description: SPA Recombinant produced in E Coli is a single non-glycosylated polypeptide chain fused with 6xHis tag at C-terminus. SPA is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 306 amino acids and having a molecular mass of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Staphylococcal Protein-A (SPA), Recombinant Protein
Full Product Name
Recombinant Staphylococcal Protein-A His Tag
Product Gene Name
Product Synonym Names
SPA, His; Staphylococcal Protein-A Recombinant, His Tag; Immunoglobulin G-binding protein A; IgG-binding protein A; Staphylococcal protein A; SPA; SPA His
Sequence
MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH< /u>
Host
E Coli
Form/Format
SPA protein was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution SPA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Molecular Weight
55,439 Da
3D Structure
ModBase 3D Structure for P38507
Solubility
It is recommended to reconstitute the lyophilized SPA in sterile 18M Omega -cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Other Notes

Small volumes of SPA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPA purchase

MBS146582 is a recombinant protein targeting SPA. Also known as Recombinant Staphylococcal Protein-A His Tag. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SPA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Immunoglobulin G-binding protein A
UniProt Protein Name
Immunoglobulin G-binding protein A
UniProt Synonym Protein Names
Staphylococcal protein A
UniProt Gene Name
spa
UniProt Synonym Gene Names
IgG-binding protein A
UniProt Entry Name
SPA_STAAU

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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