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Polymerase Delta 4 (POLD4) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146590

Description: POLD4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (1-107) and having a molecular mass of 14.8kDa.POLD4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Polymerase Delta 4 (POLD4), Recombinant Protein
Full Product Name
Recombinant Human Polymerase Delta 4
Product Gene Name
Product Synonym Names
POLD4 Human; Polymerase Delta 4 Human Recombinant; DNA polymerase delta subunit 4; p12; POLDS; DNA polymerase delta subunit p12; POLD4; Polymerase Delta 4
Sequence
MGSSHHH HHH SSGLVPRGSH MGSMGR KRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL .
Host
E Coli
Form/Format
The POLD4 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
8,868 Da
OMIM
611525
3D Structure
ModBase 3D Structure for Q9HCU8

Other Notes

Small volumes of POLD4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLD4 purchase

MBS146590 is a recombinant protein targeting POLD4. Also known as Recombinant Human Polymerase Delta 4. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLD4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase delta subunit 4 isoform 2
NCBI Official Synonym Full Names
polymerase (DNA-directed), delta 4, accessory subunit
NCBI Official Symbol
POLD4
NCBI Official Synonym Symbols
p12; POLDS
UniProt Protein Name
DNA polymerase delta subunit 4
UniProt Synonym Protein Names
DNA polymerase delta subunit p12
UniProt Gene Name
POLD4
UniProt Synonym Gene Names
POLDS
UniProt Entry Name
DPOD4_HUMAN
NCBI Protein Information
DNA polymerase delta subunit 4; DNA polymerase delta smallest subunit p12

NCBI Summary for POLD4

This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
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UniProt Comments for POLD4

POLD4: Required for optimal DNA polymerase delta activity. May contribute to PCNA-dependent activity of DNA polymerase delta. Belongs to the DNA polymerase delta subunit 4 family.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; mismatch repair; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; mitotic cell cycle; DNA repair; DNA replication; telomere maintenance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with POLD4 recombinant protein

Organs/Tissues associated with POLD4 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with POLD4 and Lung
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