Mouse LDHA Active Protein – Cat# MBS146800

Lactate Dehydrogenase A (LDHA) Mouse Active Protein

Name: LDHA Active Protein; Lactate Dehydrogenase A; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS146800
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146800

Description: LDHA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332a.a.) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Lactate Dehydrogenase A (LDHA), Active Protein

Full Product Name

Recombinant Mouse Lactate Dehydrogenase A

Product Gene Name

LDHA active protein

Product Synonym Names

LDHA Mouse; L-lactate dehydrogenase A chain; LDH-A; LDH muscle subunit; LDH-M; Lactate Dehydrogenase A Mouse Recombinant

Sequence

MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHH HHH

Host

Sf9, Baculovirus cells

Form/Format

Sterile filtered colorless solution.
LDHA protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Purity/Purification

Greater than 95.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Molecular Weight

36,499 Da

3D Structure

ModBase 3D Structure for P06151

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.

Product Categories/Family for LDHA active protein

Enzymes

Other Notes

Small volumes of LDHA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHA purchase

MBS146800 is an active protein targeting LDHA. Also known as Recombinant Mouse Lactate Dehydrogenase A. Host: Sf9, Baculovirus cells.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.

NCBI GI #

6754524

NCBI GeneID

16828

NCBI Accession #

NP_034829.1

UniProt Primary Accession #

P06151

UniProt Related Accession #

P06151

GenBank Nucleotide #

NM_010699.2

NCBI Official Full Name

L-lactate dehydrogenase A chain isoform 1

NCBI Official Synonym Full Names

lactate dehydrogenase A

NCBI Official Symbol

Ldha

NCBI Official Synonym Symbols

Ldh1; Ldhm; l7R2

UniProt Protein Name

L-lactate dehydrogenase A chain

UniProt Synonym Protein Names

LDH muscle subunit; LDH-M

Protein Family

L-lactate dehydrogenase

UniProt Gene Name

Ldha

UniProt Synonym Gene Names

Ldh-1; Ldh1; LDH-A; LDH-M

NCBI Protein Information

L-lactate dehydrogenase A chain

NCBI Summary for LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]

UniProt Comments for LDHA

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 7 B3|7 30.6 cM

Cellular Component: cell-cell adherens junction; cytosol; membrane; mitochondrion; nucleus

Molecular Function: L-lactate dehydrogenase activity; lactate dehydrogenase activity

Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate biosynthetic process from pyruvate

Research Articles on LDHA

1. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with LDHA active protein

Products by Pathway	Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Glucagon Signaling Pathway antibodies	Glucagon Signaling Pathway Diagram
Glucagon Signaling Pathway antibodies	Glucagon Signaling Pathway Diagram
Glycolysis / Gluconeogenesis Pathway antibodies	Glycolysis / Gluconeogenesis Pathway Diagram
Glycolysis / Gluconeogenesis Pathway antibodies	Glycolysis / Gluconeogenesis Pathway Diagram
Glycolysis And Gluconeogenesis Pathway antibodies	Glycolysis And Gluconeogenesis Pathway Diagram

Diseases associated with LDHA active protein

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>22 publications with LDHA and Adenocarcinoma
Liver Diseases Antibodies	>13 publications with LDHA and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>12 publications with LDHA and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>12 publications with LDHA and Breast Neoplasms
Liver Neoplasms Antibodies	>10 publications with LDHA and Liver Neoplasms
Neoplasms, Experimental Antibodies	>9 publications with LDHA and Neoplasms, Experimental
Neoplasm Metastasis Antibodies	>8 publications with LDHA and Neoplasm Metastasis
Lung Neoplasms Antibodies	>8 publications with LDHA and Lung Neoplasms
Lactate dehydrogenase deficiency type A Antibodies	>8 publications with LDHA and Lactate dehydrogenase deficiency type A
Kidney Diseases Antibodies	>7 publications with LDHA and Kidney Diseases

Organs/Tissues associated with LDHA active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>43 publications with LDHA and Blood
Liver Antibodies	>27 publications with LDHA and Liver
Muscle Antibodies	>25 publications with LDHA and Muscle
Brain Antibodies	>22 publications with LDHA and Brain
Testis Antibodies	>20 publications with LDHA and Testis
Pancreas Antibodies	>14 publications with LDHA and Pancreas
Lung Antibodies	>12 publications with LDHA and Lung
Kidney Antibodies	>12 publications with LDHA and Kidney
Bone Antibodies	>12 publications with LDHA and Bone
Heart Antibodies	>11 publications with LDHA and Heart