Staphylococcal sspA Recombinant Protein – Cat# MBS146803

Glutamyl Endopeptidase (sspA) Staphylococcal Recombinant Protein

Name: sspA Recombinant Protein; Glutamyl Endopeptidase; Staphylococcal; MyBioSource
Brand: MyBioSource
SKU: MBS146803
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146803

Description: Recombinant Staphylococcal GLU-C produced in E Coli is a single, non-glycosylated polypeptide chain containing a total of 267 amino acids and having a molecular mass of 28.9kDa.Introduction: Glutamyl endopeptidase (GLU-C) is an enzyme which… More details

$250 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Glutamyl endopeptidase (sspA), Recombinant Protein

Full Product Name

Recombinant Staphylococcal Glutamyl endopeptidase

Product Gene Name

sspA recombinant protein

Product Synonym Names

GLU-C S.aureus; Glutamyl endopeptidase (EC: 3.4.21.19); Endoproteinase Glu-C; Staphylococcal serine proteinase; V8 protease; V8 proteinase; sspA; Glutamyl endopeptidase Staphylococcal Recombinant

Sequence

MLPNNDRHQI TDTTNGHYAP VTYIQVEAPT GTFIASGVVV GKDTLLTNKH VVDATHGDPH ALKAFPSAIN QDNYPNGGFT AEQITKYSGE GDLAIVKFSP NEQNKHIGEV VKPATMSNNA ETQVNQNITV TGYPGDKPVA TMWESKGKIT YLKGEAMQYD LSTTGGNSGS PVFNEKNEVI GIHWGGVPNE FNGAVFINEN VRNFLKQNIE DIHFANDDQP NNPDNPDNPN NPDNPNNPDE PNNPDNPNNP DNPDNGDNNN SDNPDAA

Host

E Coli

Form/Format

Lyophilized from a sterile (0.2 um) filtered aqueous solution containing 10mM sodium phosphate, pH 7.5.

Purity/Purification

Greater than 95% as determined by SDS-PAGE.

Preparation and Storage

Although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GLU-C should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Molecular Weight

36,326 Da

3D Structure

ModBase 3D Structure for P0C1U8

Solubility

It is recommended to reconstitute the lyophilized GLU-C in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for sspA recombinant protein

Enzymes

Other Notes

Small volumes of sspA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for sspA purchase

MBS146803 is a recombinant protein targeting sspA. Also known as Recombinant Staphylococcal Glutamyl endopeptidase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for sspA. It may not necessarily be applicable to this product.

NCBI GI #

446599202

NCBI Accession #

WP_000676548.1

UniProt Primary Accession #

P0C1U8

UniProt Secondary Accession #

P04188

GenBank Nucleotide #

WP_000676548.1

NCBI Official Full Name

MULTISPECIES: serine protease

UniProt Protein Name

Glutamyl endopeptidase

UniProt Synonym Protein Names

Endoproteinase Glu-C; Staphylococcal serine proteinase; V8 protease; V8 proteinase

Protein Family

Glutamyl endopeptidase

UniProt Gene Name

sspA

UniProt Comments for sspA

Preferentially cleaves peptide bonds on the carboxyl-terminal side of aspartate and glutamate. Along with other extracellular proteases it is involved in colonization and infection of human tissues. Required for proteolytic maturation of thiol protease SspB and inactivation of SspC, an inhibitor of SspB. It is the most important protease for degradation of fibronectin-binding protein (FnBP) and surface protein A, which are involved in adherence to host cells. May also protect bacteria against host defense mechanism by cleaving the immunoglobulin classes IgG, IgA and IgM. May be involved in the stability of secreted lipases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with sspA recombinant protein

Reference Product	PubMed Publications
sspB recombinant protein	>70 publications with sspA and sspB