Disulfide Isomerase A4 (PDIA4) Active Protein

Name: PDIA4 Active Protein; Disulfide Isomerase A4; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS146840
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146840

Description: PDIA4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 646 amino acids (21-645 a.a.) and having a molecular weight of 72.9kDa. The PDIA4 is fused to 21 a.a. His-Tag at N-terminus and purified by… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Disulfide Isomerase A4 (PDIA4), Active Protein

Full Product Name

Recombinant Human Protein Disulfide Isomerase A4, Active

Product Gene Name

PDIA4 active protein

Product Synonym Names

PDIA4 Human, Active; Endoplasmic reticulum resident protein 72; ERP70; ERP72; Protein Disulfide Isomerase A4 Human Recombinant, Active

Sequence

MGSSHHH HHH SSGLVPRGSH MVAGAE GPDE DSSNRENAIE DEEEEEEEDD DEEEDDLEVK EENGVLVLND ANFDNFVADK DTVLLEFYAP WCGHCKQFAP EYEKIANILK DKDPPIPVAK IDATSASVLA SRFDVSGYPT IKILKKGQAV DYEGSRTQEE IVAKVREVSQ PDWTPPPEVT LVLTKENFDE VVNDADIILV EFYAPWCGHC KKLAPEYEKA AKELSKRSPP IPLAKVDATA ETDLAKRFDV SGYPTLKIFR KGRPYDYNGP REKYGIVDYM IEQSGPPSKE ILTLKQVQEF LKDGDDVIII GVFKGESDPA YQQYQDAANN LREDYKFHHT FSTEIAKFLK VSQGQLVVMQ PEKFQSKYEP RSHMMDVQGS TQDSAIKDFV LKYALPLVGH RKVSNDAKRY TRRPLVVVYY SVDFSFDYRA ATQFWRSKVL EVAKDFPEYT FAIADEEDYA GEVKDLGLSE SGEDVNAAIL DESGKKFAME PEEFDSDTLR EFVTAFKKGK LKPVIKSQPV PKNNKGPVKV VVGKTFDSIV MDPKKDVLIE FYAPWCGHCK QLEPVYNSLA KKYKGQKGLV IAKMDATAND VPSDRYKVEG FPTIYFAPSG DKKNPVKFEG GDRDLEHLSK FIEEHATKLS RTKEEL

Host

E Coli

Form/Format

Sterile filtered colorless solution.
The PDIA4 1mg/ml protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT, 0.1M NaCl and 10% glycerol.

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Molecular Weight

72,932 Da

3D Structure

ModBase 3D Structure for P13667

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Product Categories/Family for PDIA4 active protein

Enzymes

Other Notes

Small volumes of PDIA4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA4 purchase

MBS146840 is an active protein targeting PDIA4. Also known as Recombinant Human Protein Disulfide Isomerase A4, Active. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA4. It may not necessarily be applicable to this product.

NCBI GI #

4758304

NCBI GeneID

9601

NCBI Accession #

NP_004902.1

UniProt Primary Accession #

P13667

UniProt Secondary Accession #

Q549T6; A8K4K6

UniProt Related Accession #

P13667

GenBank Nucleotide #

NM_004911.4

NCBI Official Full Name

protein disulfide-isomerase A4

NCBI Official Synonym Full Names

protein disulfide isomerase family A member 4

NCBI Official Symbol

PDIA4

NCBI Official Synonym Symbols

ERP70; ERP72; ERp-72

UniProt Protein Name

Protein disulfide-isomerase A4

UniProt Synonym Protein Names

Endoplasmic reticulum resident protein 70; ER protein 70; ERp70; Endoplasmic reticulum resident protein 72; ER protein 72; ERp-72; ERp72

Protein Family

Protein disulfide-isomerase

UniProt Gene Name

PDIA4

UniProt Synonym Gene Names

ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72

NCBI Protein Information

protein disulfide-isomerase A4

NCBI Summary for PDIA4

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]

UniProt Comments for PDIA4

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: Chaperone; EC 5.3.4.1; Endoplasmic reticulum; Isomerase

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: cell surface

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; protein secretion

Research Articles on PDIA4

1. CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDIA4 active protein

Reference Product	PubMed Publications
PDIA6 active protein	>5 publications with PDIA4 and PDIA6
HSPA5 active protein	>4 publications with PDIA4 and HSPA5
CALR active protein	>1 publications with PDIA4 and CALR
ERO1LB active protein	>1 publications with PDIA4 and ERO1LB

Pathways associated with PDIA4 active protein

Products by Pathway	Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Vibrio Cholerae Infection Pathway antibodies	Vibrio Cholerae Infection Pathway Diagram
Vibrio Cholerae Infection Pathway antibodies	Vibrio Cholerae Infection Pathway Diagram

Diseases associated with PDIA4 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with PDIA4 and Inflammation
Adenocarcinoma Antibodies	>2 publications with PDIA4 and Adenocarcinoma
Liver Diseases Antibodies	>2 publications with PDIA4 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with PDIA4 and Carcinoma, Hepatocellular
Nervous System Diseases Antibodies	>1 publications with PDIA4 and Nervous System Diseases
Liver Neoplasms Antibodies	>1 publications with PDIA4 and Liver Neoplasms

Organs/Tissues associated with PDIA4 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with PDIA4 and Liver
Muscle Antibodies	>2 publications with PDIA4 and Muscle
Brain Antibodies	>2 publications with PDIA4 and Brain
Lung Antibodies	>2 publications with PDIA4 and Lung
Vascular Antibodies	>1 publications with PDIA4 and Vascular
Heart Antibodies	>1 publications with PDIA4 and Heart
Blood Antibodies	>1 publications with PDIA4 and Blood
Pancreas Antibodies	>1 publications with PDIA4 and Pancreas
Eye Antibodies	>1 publications with PDIA4 and Eye
Intestine Antibodies	>1 publications with PDIA4 and Intestine