Heparanase-1 (HPSE) Human Active Protein

This human HPSE (Heparanase-1) active protein degrades heparan sulfate side chains of heparan sulfate proteoglycans in the extracellular matrix, playing a role in ECM degradation and cell migration.

Background: Heparanase is an endo beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1, 2, 3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4, 5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1, 6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7, 8).

1. Vlodavsky, I. et al.., Nat. Med. 5 (1999) 793-802.2. Vlodavsky, I and Friedman, Y., J. Clin. Invest. 108 (2001) 341-347.3. Parish, C.R. et al., Biochem. Biophys. Acta 1471 (2001) M99-M108.4. Vlodasvsky, I. et al., Cancer Metastasis Rev. 9, (1990) 203-226.5. Bashkin, P. et al., . Biochemistry 28 (1989), 1737-1743.6. Fairbanks, M.B. et al., J. Biol. Chem. 274 (1999) 29587- 29590.7. Koliopanos, A.et al., Cancer Res. 61 (1992) 4655-4659.8. Gohji, K. et al., Int. J. Cancer 95 (2001) 295-301

Small volumes of HPSE active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS147099 is an active protein targeting HPSE. Also known as Recombinant Human Heparanase-1 Active.