Human TDP1 Recombinant Protein – Cat# MBS147105

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Human Recombinant Protein

Name: TDP1 Recombinant Protein; Tyrosyl-DNA Phosphodiesterase 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS147105
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS147105

Description: TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).… More details

$250 / 0.001 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tyrosyl-DNA Phosphodiesterase 1 (TDP1), Recombinant Protein

Full Product Name

Recombinant Human Tyrosyl-DNA Phosphodiesterase 1, Sf9

Product Gene Name

TDP1 recombinant protein

Product Synonym Names

Tyrosyl-DNA phosphodiesterase 1; Tyr-DNA phosphodiesterase 1; TDP1; FLJ11090; MGC104252; TDP1 Human, Sf9; Tyrosyl-DNA Phosphodiesterase 1 Human Recombinant, Sf9

Sequence

ADP MSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH

Host

Sf9, Baculovirus cells

Form/Format

Sterile filtered clear solution
Protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles

Molecular Weight

41,630 Da

OMIM

BX161451 mRNA

3D Structure

ModBase 3D Structure for Q9NUW8

Product Categories/Family for TDP1 recombinant protein

Enzymes; Tyrosine Kinase; TDP1 Human; Sf9

Other Notes

Small volumes of TDP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TDP1 purchase

MBS147105 is a recombinant protein targeting TDP1. Also known as Recombinant Human Tyrosyl-DNA Phosphodiesterase 1, Sf9. Host: Sf9, Baculovirus cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TDP1. It may not necessarily be applicable to this product.

NCBI GI #

37999797

NCBI GeneID

55775

NCBI Accession #

Q9NUW8.2

UniProt Primary Accession #

Q9NUW8

UniProt Secondary Accession #

Q2HXX4; Q86TV8; Q96BK7; Q9NZM7; Q9NZM8

UniProt Related Accession #

Q9NUW8

NCBI Official Full Name

Tyrosyl-DNA phosphodiesterase 1

NCBI Official Synonym Full Names

tyrosyl-DNA phosphodiesterase 1

NCBI Official Symbol

TDP1

UniProt Protein Name

Tyrosyl-DNA phosphodiesterase 1

Protein Family

Tyrosyl-DNA phosphodiesterase

UniProt Gene Name

TDP1

UniProt Synonym Gene Names

Tyr-DNA phosphodiesterase 1

NCBI Protein Information

tyrosyl-DNA phosphodiesterase 1

NCBI Summary for TDP1

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]

UniProt Comments for TDP1

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.

Research Articles on TDP1

1. We show that two genes, TDP1, a tyrosyl-DNA-phosphdiesterase, and TAF12, an RNA polymerase II TATA-box binding factor, cause CIN when overexpressed in human cells. Using SDL screens in yeast, we identified a set of genes that when deleted specifically kill cells with high levels of Tdp1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TDP1 recombinant protein

Reference Product	PubMed Publications
TOP1 recombinant protein	>51 publications with TDP1 and TOP1
XRCC1 recombinant protein	>14 publications with TDP1 and XRCC1
APTX recombinant protein	>11 publications with TDP1 and APTX
ATM recombinant protein	>8 publications with TDP1 and ATM
PNKP recombinant protein	>5 publications with TDP1 and PNKP
LIG3 recombinant protein	>1 publications with TDP1 and LIG3

Pathways associated with TDP1 recombinant protein

Products by Pathway	Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
Double-Strand Break Repair Pathway antibodies	Double-Strand Break Repair Pathway Diagram
Nonhomologous End-joining (NHEJ) Pathway antibodies	Nonhomologous End-joining (NHEJ) Pathway Diagram
Processing Of DNA Ends Prior To End Rejoining Pathway antibodies	Processing Of DNA Ends Prior To End Rejoining Pathway Diagram

Diseases associated with TDP1 recombinant protein

Disease Name	Pubmed Publications
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy Antibodies	>5 publications with TDP1 and Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
Cardiovascular Diseases Antibodies	>4 publications with TDP1 and Cardiovascular Diseases
Lung Neoplasms Antibodies	>3 publications with TDP1 and Lung Neoplasms
Disease Models, Animal Antibodies	>2 publications with TDP1 and Disease Models, Animal
Anemia Antibodies	>2 publications with TDP1 and Anemia
Necrosis Antibodies	>2 publications with TDP1 and Necrosis
Atherosclerosis Antibodies	>1 publications with TDP1 and Atherosclerosis
Cognition Disorders Antibodies	>1 publications with TDP1 and Cognition Disorders
Neoplasms, Experimental Antibodies	>1 publications with TDP1 and Neoplasms, Experimental
Inflammation Antibodies	>1 publications with TDP1 and Inflammation

Organs/Tissues associated with TDP1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with TDP1 and Brain
Lung Antibodies	>5 publications with TDP1 and Lung
Blood Antibodies	>4 publications with TDP1 and Blood
Muscle Antibodies	>3 publications with TDP1 and Muscle
Eye Antibodies	>2 publications with TDP1 and Eye
Bone Antibodies	>2 publications with TDP1 and Bone
Bone Marrow Antibodies	>2 publications with TDP1 and Bone Marrow
Skin Antibodies	>2 publications with TDP1 and Skin
Vascular Antibodies	>1 publications with TDP1 and Vascular
Spleen Antibodies	>1 publications with TDP1 and Spleen