Human ECHS1 Active Protein – Cat# MBS147113

Enoyl Coa Hydratase, Short Chain, 1 (ECHS1) Human Active Protein

Name: ECHS1 Active Protein; Enoyl Coa Hydratase, Short Chain, 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS147113
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS147113

Description: ECHS1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (28-290 a.a) and having a molecular mass of 30.6kDa. ECHS1 is fused to a 21 amino acid His-tag at N-terminus & purified by… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Enoyl CoA Hydratase, Short chain, 1 (ECHS1), Active Protein

Full Product Name

Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial, Active

Product Gene Name

ECHS1 active protein

Product Synonym Names

Enoyl-CoA Hydratase; Short Chain1; Enoyl Coenzyme A Hydratase; Short Chain; 1, mitochondrial; Enoyl-CoA Hydratase; Short Chain; 1, mitochondrial; Short Chain Enoyl-CoA Hydratase; EC 4.2.1.17; SCEH; Enoyl-CoA Hydratase, mitochondrial; Short-Chain Enoyl-CoA Hydratase; Enoyl-CoA Hydratase 1; EC 4.2.1; ECHS1D; ECHS1 ; ECHS1 Human, Active; Enoyl CoA Hydratase, Short chain, 1, Mitochondrial, Human Recombinant, Active

Sequence

MGSSHHHHHH SSGLVPRGSH M ASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

Host

Escherichia Coli

Form/Format

Sterile filtered clear solution
Protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% Glycerol and 100mM NaCl

Purity/Purification

Greater than 95.0% as determined by SDS-PAGE

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles

Molecular Weight

31,387 Da

OMIM

D13900 mRNA

3D Structure

ModBase 3D Structure for P30084

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25 degree C

Product Categories/Family for ECHS1 active protein

Enzymes; Hydratase; ECHS1 Human; Active

Other Notes

Small volumes of ECHS1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ECHS1 purchase

MBS147113 is an active protein targeting ECHS1. Also known as Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial, Active. Host: Escherichia Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ECHS1. It may not necessarily be applicable to this product.

NCBI GI #

62906863

NCBI GeneID

1892

NCBI Accession #

P30084.4

UniProt Primary Accession #

P30084

UniProt Secondary Accession #

O00739; Q5VWY1; Q96H54

UniProt Related Accession #

P30084

NCBI Official Full Name

Enoyl-CoA hydratase, mitochondrial

NCBI Official Synonym Full Names

enoyl-CoA hydratase, short chain 1

NCBI Official Symbol

ECHS1

NCBI Official Synonym Symbols

SCEH; ECHS1D

UniProt Protein Name

Enoyl-CoA hydratase, mitochondrial

UniProt Synonym Protein Names

Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH

Protein Family

Enoyl-CoA hydratase

UniProt Gene Name

ECHS1

UniProt Synonym Gene Names

SCEH

NCBI Protein Information

enoyl-CoA hydratase, mitochondrial

NCBI Summary for ECHS1

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

UniProt Comments for ECHS1

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.

Research Articles on ECHS1

1. exceeding nutrients suppress Enoyl-CoA hydratase-1 (ECHS1) activity by inducing its acetylation resulting in accumulation of fatty acids and branched-chain amino acids and oncogenic mTOR activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ECHS1 active protein

Reference Product	PubMed Publications
ACADS active protein	>4 publications with ECHS1 and ACADS
HADH active protein	>3 publications with ECHS1 and HADH
ACADM active protein	>2 publications with ECHS1 and ACADM
GCDH active protein	>1 publications with ECHS1 and GCDH
ACADVL active protein	>1 publications with ECHS1 and ACADVL
ACAD8 active protein	>1 publications with ECHS1 and ACAD8
HSD17B10 active protein	>1 publications with ECHS1 and HSD17B10

Pathways associated with ECHS1 active protein

Products by Pathway	Pathway Diagram
Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway antibodies	Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway Diagram
Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway antibodies	Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway Diagram
Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway antibodies	Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway Diagram
Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway antibodies	Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway Diagram
Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway antibodies	Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram

Diseases associated with ECHS1 active protein

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>15 publications with ECHS1 and Fatty Liver
Liver Diseases Antibodies	>15 publications with ECHS1 and Liver Diseases
Nervous System Diseases Antibodies	>10 publications with ECHS1 and Nervous System Diseases
MITOCHONDRIAL SHORT-CHAIN ENOYL-CoA HYDRATASE 1 DEFICIENCY Antibodies	>9 publications with ECHS1 and MITOCHONDRIAL SHORT-CHAIN ENOYL-CoA HYDRATASE 1 DEFICIENCY
Leigh Disease Antibodies	>9 publications with ECHS1 and Leigh Disease
Liver Neoplasms Antibodies	>7 publications with ECHS1 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>7 publications with ECHS1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>5 publications with ECHS1 and Inflammation
Obesity Antibodies	>4 publications with ECHS1 and Obesity
Hyperplasia Antibodies	>2 publications with ECHS1 and Hyperplasia

Organs/Tissues associated with ECHS1 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>23 publications with ECHS1 and Liver
Brain Antibodies	>10 publications with ECHS1 and Brain
Blood Antibodies	>8 publications with ECHS1 and Blood
Muscle Antibodies	>4 publications with ECHS1 and Muscle
Testis Antibodies	>2 publications with ECHS1 and Testis
Thyroid Antibodies	>2 publications with ECHS1 and Thyroid
Prostate Antibodies	>2 publications with ECHS1 and Prostate
Nerve Antibodies	>2 publications with ECHS1 and Nerve
Skin Antibodies	>2 publications with ECHS1 and Skin
Adipose Tissue Antibodies	>2 publications with ECHS1 and Adipose Tissue