Escherichia Coli hsdM Recombinant Protein – Cat# MBS1471451

Type I Restriction Enzyme Ecoei M Protein (hsdM) Escherichia Coli Recombinant Protein

Name: hsdM Recombinant Protein; Escherichia Coli; MyBioSource
Brand: MyBioSource
SKU: MBS1471451
Price: 1285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1471451

MBS1471451 is a recombinant protein targeting hsdM. Also known as Recombinant Escherichia coli Type I restriction enzyme EcoEI M protein (hsdM). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1285 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Type I restriction enzyme EcoEI M protein (hsdM), Recombinant Protein

Full Product Name

Recombinant Escherichia coli Type I restriction enzyme EcoEI M protein (hsdM)

Product Gene Name

hsdM recombinant protein

Product Synonym Gene Name

hsdM

Sequence

MSISSVIKSL QDIMRKDAGV DGDAQRLGQL SWLLFLKIFD TQEEELELEQ DDYQFPIPQR YLWRSWAANS EGITGDALLE FVNDDLFPTL KNLTAPIDKN PRGFVVKQAF SDAYNYMKNG TLLRQVINKL NEIDFSSSQE RHLFGDIYEQ ILRDLQSAGN AGEFYTPRAV TRFMVNRIDP KLGESIMDPA CGTGGFLACA FDHVKDNYVK TTEDHKTLQQ QIYGVEKKQL PHLLCTTNML LHGIEVPVQI RHDNTLNKPL SSWDEQVDVI VTNPPFGGTE EDGIEKNFPA EMQTRETADL FLQLIIEVLA DKGRAAVVLP DGTLFGEGVK TKIKKLLTEE CNLHTIVRLP NGVFNPYTGI KTNILFFTKG QPTKEVWFYE HPYPDGVKNY SKTKPMKFEE FQAEIDWWGN EADDFASREE NNQAWKVGID DIIARNFNLD IKNPYQGETI SHDPDELLAQ YQTQQAEIGE LRNQLRDILG AALAGNKGAN

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

55,620 Da

Sequence Positions

1-490, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for Q47282

Species

Escherichia coli

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of hsdM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for hsdM. It may not necessarily be applicable to this product.

NCBI GI #

970265608

NCBI Accession #

WP_058649287.1

UniProt Primary Accession #

Q47282

GenBank Nucleotide #

WP_058649287.1

NCBI Official Full Name

MULTISPECIES: restriction endonuclease subunit M

UniProt Protein Name

Type I restriction enzyme EcoEI M protein

Protein Family

Type I restriction enzyme EcoEI M protein

UniProt Gene Name

hsdM

UniProt Synonym Gene Names

M.EcoEI

UniProt Comments for hsdM

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoEI enzyme recognizes 5'-GAGN7ATGC-3'.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.