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Sclerostin (SOST) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS147270

Description: SOST Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 24-213) containing 196 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 22.4kDa (calculated). Introduction: Sclerostin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sclerostin (SOST), Recombinant Protein
Full Product Name
Recombinant Human Sclerostin, HEK
Product Gene Name
Product Synonym Names
Sclerostin; SOST; CDD; VBCH; SOST Human, HEK; Sclerostin Human Recombinant, HEK
Sequence
QGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY HHHHHH
Host
HEK293 Cells
Form/Format
Filtered white lyophilized (freeze-dried) powder
Filtered (0.4 um) and lyophilized from 0.5mg/ml in PBS and 5 % (w/v) trehalose, pH 7.4.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE
Preparation and Storage
Store lyophilized protein at-20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after one week at 4 degree C
Molecular Weight
24,264 Da
OMIM
AF331844 mRNA
3D Structure
ModBase 3D Structure for Q9BQB4
Solubility
It is recommended to add 200ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. SOST is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture

Other Notes

Small volumes of SOST recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOST purchase

MBS147270 is a recombinant protein targeting SOST. Also known as Recombinant Human Sclerostin, HEK. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for SOST. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
SOST
NCBI Official Synonym Symbols
CDD; VBCH; DAND6; SOST1
UniProt Protein Name
Sclerostin
Protein Family
UniProt Gene Name
SOST
NCBI Protein Information
sclerostin

NCBI Summary for SOST

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOST

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SOST recombinant protein

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