Human COBRA1 Recombinant Protein – Cat# MBS1482044

Negative Elongation Factor B (COBRA1) Human Recombinant Protein

Name: COBRA1 Recombinant Protein; Negative Elongation Factor B; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1482044
Price: 1370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1482044

MBS1482044 is a recombinant protein targeting COBRA1. Also known as Recombinant Human Negative elongation factor B (COBRA1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1370 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Negative elongation factor B (COBRA1), Recombinant Protein

Full Product Name

Recombinant Human Negative elongation factor B (COBRA1)

Product Gene Name

COBRA1 recombinant protein

Product Synonym Gene Name

COBRA1

Sequence

MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ LDHRKPSPAQ AAETPALELP LPSVPAPAPL

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

65,697 Da

Sequence Positions

1-580, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

611180

3D Structure

ModBase 3D Structure for Q8WX92

Species

Human

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of COBRA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COBRA1. It may not necessarily be applicable to this product.

NCBI GI #

862669351

NCBI GeneID

25920

NCBI Accession #

NP_056271.3

UniProt Primary Accession #

Q8WX92

UniProt Secondary Accession #

Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3

UniProt Related Accession #

Q8WX92

GenBank Nucleotide #

NM_015456.4

NCBI Official Full Name

negative elongation factor B

NCBI Official Synonym Full Names

negative elongation factor complex member B

NCBI Official Symbol

NELFB

NCBI Official Synonym Symbols

COBRA1; NELF-B

UniProt Protein Name

Negative elongation factor B

UniProt Synonym Protein Names

Cofactor of BRCA1

UniProt Gene Name

NELFB

UniProt Synonym Gene Names

COBRA1; KIAA1182; NELF-B

NCBI Protein Information

negative elongation factor B

NCBI Summary for COBRA1

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

UniProt Comments for COBRA1

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

Research Articles on COBRA1

1. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with COBRA1 recombinant protein

Reference Product	PubMed Publications
BRCA1 recombinant protein	>12 publications with COBRA1 and BRCA1
JUN recombinant protein	>1 publications with COBRA1 and JUN
FOS recombinant protein	>1 publications with COBRA1 and FOS

Pathways associated with COBRA1 recombinant protein

Products by Pathway	Pathway Diagram
Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies	Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies	Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies	Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
Formation Of RNA Pol II Elongation Complex Pathway antibodies	Formation Of RNA Pol II Elongation Complex Pathway Diagram
Formation Of The Early Elongation Complex Pathway antibodies	Formation Of The Early Elongation Complex Pathway Diagram
Formation Of The HIV-1 Early Elongation Complex Pathway antibodies	Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram

Diseases associated with COBRA1 recombinant protein

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>6 publications with COBRA1 and Breast Neoplasms
Adenocarcinoma Antibodies	>4 publications with COBRA1 and Adenocarcinoma
Pregnancy Complications Antibodies	>1 publications with COBRA1 and Pregnancy Complications
Prostatic Diseases Antibodies	>1 publications with COBRA1 and Prostatic Diseases
Ovarian Diseases Antibodies	>1 publications with COBRA1 and Ovarian Diseases
Gonadal Disorders Antibodies	>1 publications with COBRA1 and Gonadal Disorders
Prostatic Neoplasms Antibodies	>1 publications with COBRA1 and Prostatic Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with COBRA1 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with COBRA1 and Liver Neoplasms
Liver Diseases Antibodies	>1 publications with COBRA1 and Liver Diseases

Organs/Tissues associated with COBRA1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>1 publications with COBRA1 and Nerve
Mammary Gland Antibodies	>1 publications with COBRA1 and Mammary Gland
Liver Antibodies	>1 publications with COBRA1 and Liver
Stomach Antibodies	>1 publications with COBRA1 and Stomach
Embryonic Tissue Antibodies	>1 publications with COBRA1 and Embryonic Tissue
Kidney Antibodies	>1 publications with COBRA1 and Kidney
Prostate Antibodies	>1 publications with COBRA1 and Prostate
Brain Antibodies	>1 publications with COBRA1 and Brain