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Alpha-Enolase (ENO1B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1489591

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses By similarity. May also function in the intravascular and pericellular fibrinolytic system due… More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha-enolase (Eno1b), Polyclonal Antibody
Full Product Name
Rabbit anti-mouse Alpha-enolase polyclonal Antibody
Product Gene Name
Product Synonym Names
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; Eno1; Eno-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Human. Other species are not tested. Please decide the specificity by homology.
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Matching Pairs
Unconjugated Antibody: Alpha-enolase (MBS1489591)
FITC Conjugated Antibody: Alpha-enolase (MBS1489209)
Matching Pairs
Unconjugated Antibody: Alpha-enolase (MBS1489591)
HRP Conjugated Antibody: Alpha-enolase (MBS1490673)
Molecular Weight
47,141 Da
3D Structure
ModBase 3D Structure for P17182
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant mouse Alpha-enolase protein
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Eno1b antibody
ELISA (EIA), Western Blot (WB)

Western Blot (WB) of anti-Eno1b antibody

Western blot
All lanes: Alpha-enolase antibody at 2ug/ml
Lane 1:PC-3 whole cell lysate
Lane 2:mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 73kDa
Observed band size: 73kDa
Rabbit Anti-ENO1B Polyclonal Antibody – Cat# MBS1489591

Western Blot (WB) of anti-Eno1b antibody

Western Blot
Positive WB detected in: hepG2 cell,mouse heart,mouse liver,mouse spleen,mouse lung,mouse kidney,mouse brain,mouse skeletal muscle
All lanes: Eno1 antibody at 2.6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47kDa
Observed band size: 47 kDa
Rabbit Anti-ENO1B Polyclonal Antibody, Western Blot (WB) – Cat# MBS1489591

Other Notes

Small volumes of anti-Eno1b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Eno1b purchase

MBS1489591 is an antibody targeting Eno1b. Also known as Rabbit anti-mouse Alpha-enolase polyclonal Antibody. Reactive in Mouse, Human. Other species are not tested. Please decide the specificity by homology.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Eno1b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
enolase 1B, retrotransposed
NCBI Official Synonym Full Names
enolase 1B, retrotransposed
NCBI Official Symbol
Eno1b
NCBI Official Synonym Symbols
Eno1; Gm5506; EG433182
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
Protein Family
UniProt Gene Name
Eno1
UniProt Synonym Gene Names
Eno-1; NNE
UniProt Entry Name
ENOA_MOUSE
NCBI Protein Information
enolase 1B, retrotransposed

NCBI Summary for Eno1b

This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1, alpha non-neuron) gene, which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved, compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq, Jul 2008]
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UniProt Comments for Eno1b

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Transcription, coactivator/corepressor

Cellular Component: cytoplasm; extracellular space; extrinsic to plasma membrane; membrane; neuron projection; nucleus

Molecular Function: GTPase binding; heat shock protein binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity; RNA binding

Biological Process: in utero embryonic development; positive regulation of binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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