Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: GFAP antibody at 3.9ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
IHC image of MBS1489669 diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0).
Section was blocked with 10% normal goat serum 30min at RT.
Then primary antibody (1% BSA) was incubated at 4°C overnight.
The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of MBS1489669 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0).
Section was blocked with 10% normal goat serum 30min at RT.
Then primary antibody (1% BSA) was incubated at 4°C overnight.
The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with MBS1489669 at 1:66, counter-stained with DAPI.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum.
The cells were then incubated with the antibody overnight at 4°C.
The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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