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Myelin-Associated Glycoprotein (MAG) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1490028

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Myelin-associated glycoprotein (MAG), Polyclonal Antibody
Full Product Name
Rabbit anti-human Myelin-associated glycoprotein polyclonal Antibody
Product Gene Name
anti-MAG antibody
Product Synonym Names
Myelin-associated glycoprotein (Siglec-4a), MAG, GMA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity/Purification
>95%, Protein G purified
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze
Matching Pairs
Unconjugated Antibody: Myelin-associated glycoprotein (MBS1490028)
Biotin Conjugated Antibody: Myelin-associated glycoprotein (MBS1488790)
Matching Pairs
Unconjugated Antibody: Myelin-associated glycoprotein (MBS1490028)
FITC Conjugated Antibody: Myelin-associated glycoprotein (MBS1490155)
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OMIM
159460
3D Structure
ModBase 3D Structure for P20916
Conjugate
Non-conjugated
Species
Human
Research Area
Neuroscience
Immunogen
Recombinant Human Myelin-associated glycoprotein (25-516AA)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9543 / sc-9542 / sc-9544 / sc-15324
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MAG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-MAG antibody
Recommended dilution:
WB: 1:2000-1:10000
IHC: 1:500-1:1000
IF: 1:50-1:500

Western Blot (WB) of anti-MAG antibody

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate
All lanes: MAG antibody at 4.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70,64,67 KDa
Observed band size: 70 KDa
Rabbit Anti-MAG Polyclonal Antibody – Cat# MBS1490028

Immunofluorescence (IF) of anti-MAG antibody

Immunofluorescence staining of MCF-7 cells with MBS1490028 at 1:416,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Rabbit Anti-MAG Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1490028

Western Blot (WB) of anti-MAG antibody

Western Blot
Positive WB detected in: SH-SY5Y whole cell
lysate, Rat brain tissue
All lanes:MAG antibody at 5mg/ml
Secondary Goat polyclonal to
rabbit IgG at 1/50000 dilution
Predicted band size: 70, 64, 67 kDa
Observed band size: 70, 100 kDa
Rabbit Anti-MAG Polyclonal Antibody, Western Blot (WB) – Cat# MBS1490028

Immunohistochemistry (IHC) of anti-MAG antibody

IHC image of MBS1490028 diluted at1:600 and staining in paraffin-embedded humankidney tissue performed on a Leica BondTMsystem. After dewaxing and hydration, antigen retrieval was mediated by high pressure in acitrate buffer (pH 6.0). Section was blocked with10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at4°C overnight. The primary is detected by abiotinylated secondary antibody and visualizedtissue using an HRP conjugated SP system.
Rabbit Anti-MAG Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1490028

Immunohistochemistry (IHC) of anti-MAG antibody

IHC image of MBS1490028 diluted at1:600 and staining in paraffin-embedded humanbrain tissue performed on a Leica BondTMsystem. After dewaxing and hydration, antigenretrieval was mediated by high pressure in acitrate buffer (pH 6.0). Section was blocked with10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at4°C overnight. The primary is detected by abiotinylated secondary antibody and visualizedtissue using an HRP conjugated SP system
Rabbit Anti-MAG Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1490028

Other Notes

Small volumes of anti-MAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAG purchase

MBS1490028 is an antibody targeting MAG. Also known as Rabbit anti-human Myelin-associated glycoprotein polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAG. It may not necessarily be applicable to this product.

NCBI GI #
312836851
NCBI GeneID
4099
NCBI Accession #
NP_001186145.1
UniProt Primary Accession #
P20916
UniProt Secondary Accession #
Q567S4; B7Z2E5; F5GYC0
UniProt Related Accession #
P20916
GenBank Nucleotide #
NM_001199216.1
NCBI Official Full Name
myelin-associated glycoprotein isoform c
NCBI Official Synonym Full Names
myelin associated glycoprotein
NCBI Official Symbol
MAG
NCBI Official Synonym Symbols
GMA; S-MAG; SIGLEC4A; SIGLEC-4A
UniProt Protein Name
Myelin-associated glycoprotein
UniProt Synonym Protein Names
Siglec-4a
Protein Family
Allergen
UniProt Gene Name
MAG
UniProt Synonym Gene Names
GMA
UniProt Entry Name
MAG_HUMAN
NCBI Protein Information
myelin-associated glycoprotein

NCBI Summary for MAG

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]
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UniProt Comments for MAG

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; integral to membrane; paranode region of axon; plasma membrane

Molecular Function: carbohydrate binding; protein kinase binding; receptor binding

Biological Process: blood coagulation; cell adhesion; leukocyte migration; negative regulation of axonogenesis; nerve growth factor receptor signaling pathway; positive regulation of astrocyte differentiation; positive regulation of myelination; regulation of axonogenesis; substantia nigra development
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Research Articles on MAG

1. This study identify involvement of myelin-associated glycoprotein in this family with a disorder affecting the central and peripheral nervous system, and suggest that loss of the protein function is responsible for the unique clinical phenotype

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAG antibody

 Reference Product  PubMed Publications
 MBP antibody  >195 publications with MAG and MBP
 RTN4R antibody  >50 publications with MAG and RTN4R
 RHOA antibody  >33 publications with MAG and RHOA
 OMG antibody  >25 publications with MAG and OMG
 LINGO1 antibody  >8 publications with MAG and LINGO1
 NGFR antibody  >4 publications with MAG and NGFR
 RTN4 antibody  >2 publications with MAG and RTN4

Pathways associated with anti-MAG antibody

 Products by Pathway  Pathway Diagram
 Axonal Growth Inhibition (RHOA Activation) Pathway antibodies  Axonal Growth Inhibition (RHOA Activation) Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Glial Cell Differentiation Pathway antibodies  Glial Cell Differentiation Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signalling By NGF Pathway antibodies  Signalling By NGF Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-MAG antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >892 publications with MAG and Immune System Diseases
 Brain Diseases Antibodies  >316 publications with MAG and Brain Diseases
 Kidney Diseases Antibodies  >171 publications with MAG and Kidney Diseases
 Disease Models, Animal Antibodies  >146 publications with MAG and Disease Models, Animal
 Hemorrhage Antibodies  >144 publications with MAG and Hemorrhage
 Liver Diseases Antibodies  >143 publications with MAG and Liver Diseases
 Pain Antibodies  >122 publications with MAG and Pain
 Inflammation Antibodies  >119 publications with MAG and Inflammation
 Hypertension Antibodies  >81 publications with MAG and Hypertension
 Necrosis Antibodies  >69 publications with MAG and Necrosis

Organs/Tissues associated with anti-MAG antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2929 publications with MAG and Blood
 Nerve Antibodies  >752 publications with MAG and Nerve
 Brain Antibodies  >711 publications with MAG and Brain
 Kidney Antibodies  >292 publications with MAG and Kidney
 Spleen Antibodies  >157 publications with MAG and Spleen
 Ganglia Antibodies  >97 publications with MAG and Ganglia
 Uterus Antibodies  >18 publications with MAG and Uterus
 Ovary Antibodies  >16 publications with MAG and Ovary
 Testis Antibodies  >6 publications with MAG and Testis
 Mammary Gland Antibodies  >2 publications with MAG and Mammary Gland
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